Method for increasing productivity of secondary metabolite by conferring drug-resistant mutations
AssigneesNational Food Research Institute, Kozo Ochi, Astellas Pharma Inc.
InventorsKozo Ochi, Haifeng Hu, Motohiro Hino, Akihiko Fujie, Hideyuki Muramatsu
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Abstract
This invention relates to a method for increasing the productivity of the secondary metabolite in a micro-organism, for example, the improvement of antibiotic producers by conferring drug-resistant mutations to micro-organisms.
Claims20
A method for increasing a productivity of a secondary metabolite in a microorganism by conferring a resistance against two or more antibiotics to said microorganism.
A method for obtaining a microorganism having an increased productivity of a secondary metabolite, which comprises the steps of conferring a resistance against two or more antibiotics to said microorganism by culturing it in a medium containing the antibiotics, wherein the concentration of said antibiotics is higher than MIC of said antibiotics against the original microorganism, and isolating colonies which can grow in the medium.
A method for producing a secondary metabolite, which comprises the steps of
- culturing in a medium a microorganism having a resistance against at least two antibiotics and having an increased productivity of the secondary metabolite in comparison to the original microorganism thereof,
- forming and accumulating a secondary metabolite, and
- recovering the secondary metabolite therefrom.
A method for increasing a productivity of a secondary metabolite in a microorganism by conferring a resistance against a single antibiotic, excepting streptomycin, to said microorganism.
Dependencies are inferred from the claim text.