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OwnerCsilla Ozvegy, Gergely Szakacs +2inventors
InventorsCsilla Ozvegy, Gergely Szakacs, Andras Varandi, Zoltan Nagy
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The overexpression of an ABC half-transporter, ABCG2 (MXR/BCRP) transporter causes multidrug resistance in tumors. The present invention is based on the in vitro expression of the ABCG2 protein in insect cells as an expression system free of other, closely related, functional ABC transporters. In particular, the invention is related to a method for testing drugs for their effect on ABCG2 protein, method for the expression of active ABCG2, isolated ABCG2 homooligomers, preferably homodimers and uses thereof for testing drugs. The invention is further related to cell membrane preparations and cells, preferably insect cells comprising active ABCG2 and reagent kits for testing drugs for their effect on an ABCG2 half transporter protein. The invention is also related to a method for identifying ABCG2 activity in a biological sample, utilizing its substrate/inhibitor specificity.
Method for testing drugs for their effect on ABCG2 protein, comprising
Isolated ABCG2 homooligomer, preferably homodimer said homooligomer or homodimer preferably being obtained by expression in insect cells.
Insect cell membrane preparation comprising active ABCG2, preferably of human origin, embedded in the membrane.
Insect cell comprising active ABCG2.
Reagent kit for testing drugs for their effect on an ABCG2 half transporter protein, comprising at least means for expressing an ABCG2 protein in insect cells, preferably a nucleic acid encoding ABCG2 protein, and a vector for introducing said nucleic acid into the insect cells and expressing it therein.
A method for identifying ABCG2 activity in a biological sample, comprising
Dependencies are inferred from the claim text.