Methods for using combinatorial libraries of monomer domains
AssigneeAvidia Research Institute
InventorsJoost A. Kolkman, Willem P.C. Stemmer, Per-Ola Freskgard
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Abstract
Methods for identifying discrete monomer domains and immuno-domains with a desired property are provided. Methods for generating multimers from two or more selected discrete monomer domains are also provided, along with methods for identifying multimers possessing a desired property. Presentation systems are also provided which present the discrete monomer and/or immuno-domains, selected monomer and/or immuno-domains, multimers and/or selected multimers to allow their selection. Compositions, libraries and cells that express one or more library member, along with kits and integrated systems, are also included in the present invention.
Claims24
A method for screening a library of monomer domains or multimers comprising monomer domains for binding affinity to multiple ligands, the method comprising
- contacting a library of monomer domains or multimers of monomer domains to multiple ligands;
- and
- selecting monomer domains or multimers that bind to at least one of the ligands.
The method of claim 12, wherein the polypeptides are expressed on the surface of phage.
The method of claim 23, further comprising amplifying the polynucleotides to produce a polynucleotide hybrid comprising polynucleotides from the target ligand phage and the library phage.
Dependencies are inferred from the claim text.