Methods for monitoring drug activities in vivo
OwnerMichael E. Burczynski, Natalie C. Twine +2inventors
InventorsMichael E. Burczynski, Natalie C. Twine, Andrew J. Dorner, William L. Trepicchio
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Abstract
Methods, systems and equipment useful for monitoring in vivo activities of CCI-779 or other drugs. Numerous drug activity genes can be identified by the present invention. The expression profiles of these genes in peripheral blood mononuclear cells can be modulated by CCI-779 or other drugs. Therefore, these genes can be used as surrogate markers for monitoring drug activities in vivo.
Claims20
A method comprising comparing an expression profile of at least one gene in a peripheral blood sample of a patient to a reference expression profile of said at least one gene, wherein said at least one gene is differentially expressed in peripheral blood mononuclear cells (PBMCs) of patients who have a non-blood disease and are subject to a drug therapy as compared to PBMCs isolated from said patients before said drug therapy, and wherein the patient has the non-blood disease and is being treated by said drug therapy.
A method for identifying drug activity genes, comprising:
- detecting an expression profile of genes in peripheral blood samples of patients who have a non-blood disease and are subject to a drug therapy;
- and
- comparing said expression profile to a baseline expression profile of said genes in peripheral blood samples isolated from said patients before said drug therapy so as to identify drug activity genes whose expression levels in peripheral blood samples can be modulated by said drug therapy.
A kit comprising a plurality of polynucleotides, wherein each of said polynucleotides is capable of hybridizing under stringent or nucleic acid array hybridization conditions to an RNA transcript, or the complement thereof, of a different respective gene selected from Table 5.
A kit comprising a plurality of antibodies, wherein each of said antibodies is capable of binding to a polypeptide encoded by a different respective gene selected from Table 5.
A nucleic acid array comprising polynucleotide probes, wherein a substantial portion of all polypeptide probes on the nucleic acid array can hybridize under stringent or nucleic acid array hybridization conditions to RNA transcripts, or the complements thereof, of genes selected from Table 5.
Dependencies are inferred from the claim text.