Combinational usage of insulators and coat color marker for high efficiency transgene expression and visual genotyping in mice
AssigneeLevel Biotechnology Inc.
InventorsTing-Fen Tsai, Yi-Chun Hsiao, Hsiao-Hui Chang
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Abstract
A method of identifying a genotype of a transgenic mouse and reducing the variability of a transagene expression in the transgenic mouse comprises the step of introducing into a mouse (a) a transgene expression cassette, (b) a coat color expression cassette and (c) an insulator positioned at 5′ or 3′ end of the transgene expression cassette.
Claims8
A method of identifying a genotype of a transgenic mouse and reducing the variability of a transgene expression in said transgenic mouse, comprising the steps of:
- a) introducing into the genome of a mouse a vector comprising a dominant coat color marker containing an expression cassette K14-Ag, a transgene expression cassette having RNA polymerase II large subunit promoter, and a chicken beta-globin HS4 insulator;
- and
- b) selecting a mouse for transgenesis by visually detecting a phenotype exhibiting coat color resulting from said expression cassette K14-Ag.
A transgenic mouse made by introducing into the genome of a mouse a vector comprising a dominant coat color marker containing an expression cassette K14-Ag, a transgene expression cassette having RNA polymerase II large subunit promoter, and a chicken beta-globin HS4 insulator.
A vector for identifying a genotype of a transgenic mouse and reducing the variability of a transagene expression in said transgenic mouse, comprising a dominant coat color marker containing an expression cassette K14-Ag, a transgene expression cassette having RNA polymerase II large subunit promoter, and a chicken beta-globin HS4 insulator.
Dependencies are inferred from the claim text.