Back to results
US 2004/0175723 A1Published application
TaqManTM-PCR for the detection of pathogenic E.coli strains
OwnerKlaus Pfefferinventor
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method for the detection of pathogenic E. coli in a sample comprising PCR amplification of DNA isolated from said sample using oligonucleotide primers specific for pathogenic E. coli.
Claims10
A method for the detection of pathogenic E. coli in a sample comprising PCR amplification of DNA isolated from said sample using a set of oligonucleotide primers specific for virulence factors/toxins of pathogenic E. coli selected from
- primers that hybridise to a gene encoding heat labile toxin, or heat stabile toxin for the amplification of a DNA sequence characteristic for enterotoxigenic E. coli;
- primers that hybridise to a gene encoding heat stabile toxin for the amplification of a DNA sequence characteristic for enteroaggregative E. coli;
- primers that hybridise to the pCVD432 plasmid for the amplification of a DNA sequence characteristic for enteroaggregative E. coli;
- primers that hybridise to the inv-plasmid for the amplification of a DNA sequence contained in enteroinvasive E. coli;
- primers that hybridise to the EAF plasmid, or the eae gene for the amplification of a DNA sequence characteristic for enteropathogenic E. coli;
- and/or
- primers that hybridise to the genes encoding shiga-like toxin sltI or sltII for the amplification of a DNA sequence characteristic for enterohemorrhagic E. coli, followed by detection and identification of the amplified product using conventional methods.
4 more elements
A set of primers useful for PCR amplification of DNA specific for virulence factors/toxins of pathogenic E. coli selected from:
- a set of primers that hybridise to a gene encoding heat labile toxin, or heat stabile toxin of enterotoxigenic E. coli;
- a set of primers that hybridise to a gene encoding heat stabile toxin of enteroaggregative E. coli;
- a set of primers that hybridise to the pCVD432 plasmid of enteroaggregative E. coli;
- a set of primers that hybridise to the inv-plasmid of enteroinvasive E. coli;
- a set of primers that hybridise to the EAF plasmid, or the eae gene of enteropathogenic E. coli;
- and
- a set of primers that hybridise to the gene encoding shiga-like toxin sltl or sltII of enterohemorrhagic E. coli,
4 more elements
Dependencies are inferred from the claim text.