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OwnerShankar Balasubramanian, David Klenerman +2inventors
InventorsShankar Balasubramanian, David Klenerman, Colin Barnes, Alan Rowe Williamson
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The present invention concerns a method for determining the identity of one or more single nucleotide polymorphisms (SNP) in a genome, comprising: (i) fragmenting a sample genome; (ii) contacting the fragments with an excess of a plurality of different oligonucleotide primers under conditions that permit a primer to form a duplex with a complementary region on a fragment, each primer having a predetermined sequence complementary to a sequence on the genome that is proximal to a putative SNP site, and the resulting duplexes being immobilised on a solid support; (iii) carrying out the sequencing reaction(s) and detecting the incorporation of bases onto the oligonucleotide primers to extend the primers to at least the SNP site; and (iv) comparing the resulting sequences to those of the reference one or more SNPs.
A method for determining the identity of one or more single nucleotide polymorphisms (SNP) in a genome, comprising:
A method according to any preceding claim, wherein step (ii) comprises between 300 to 106 different oligonucleotide primers.
A method according to any preceding claim, wherein step (ii) comprises from 103 to 105 different oligonucleotide primers.
A method according to any preceding claim, wherein step (ii) comprises from 103 to 104 different oligonucleotide primers.
A method according to any preceding claim, wherein the oligonucleotide primers comprise from 10 to 70 bases.
A method according to any preceding claim, wherein the oligonucleotide primers comprise from 30 to 50 bases.
A method according to any preceding claim, wherein the oligonucleotide primers comprise about 40 bases.
A method according to any preceding claim, wherein the primers are complementary to a sequence less than 20 bases from the SNP site.
A method according to any preceding claim, wherein the primers are complementary to a sequence less than 10 bases from the SNP site.
A method according to any preceding claim, wherein the primers are complementary to a sequence from 1 to 6 bases from the SNP site.
A method according to any preceding claim, wherein the primers are complementary to a sequence adjacent to the SNP site.
A method according to any preceding claim, wherein step (iii) comprises the sequential addition of fluorescently-labelled bases.
Dependencies are inferred from the claim text.