Method and device for simultaneously molecularly cloning and polylocus profiling of genomes or genomes mixtures
OwnerLeigh A. Burgoyneinventor
Life of this patent
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Abstract
A method for amplifying genetic material by amplifying the genetic material using a single primer sequence. A detector for detecting pathogens in a sample, the detector including a single primer sequence for use in amplification reaction whereby the primer sequence amplifies genetic material of a pathogen thereby detecting pathogens in a sample. A kit for performing the above method including a single primer sequence and a device for amplifying genetic material. A computer program for creating the primers for use in the above methods.
Claims21
A method for amplifying genetic material by amplifying the genetic material using a single primer sequence.
A method for detecting the presence of pathogens in a sample by:
- amplifying genetic material for a pathogen in the sample using a single primer in an amplification process.
A detector for detecting pathogens in a sample, said detector comprising a single primer sequence for use in an amplification reaction, whereby said primer sequence amplifies pathogen genetic material thereby detecting pathogens in a sample.
A method for amplifying unknown genetic material in a sample by:
- adding a single primer sequence to the sample thereby preventing primer-dimers and primer concatenates;
- amplifying the genetic material with multiple long extension times thereby enabling highly specific amplification of the unknown genetic material.
A method for amplifying unknown genetic material in a sample by:
- adding a single primer sequence to the sample thereby preventing primer-dimers and primer concatenates;
- and
- amplifying the genetic material with multiple short extension times thereby enabling highly specific amplification of the unknown genetic material.
A method for amplifying unknown genetic material in a sample by:
- collecting the sample;
- maintaining the sample in storage media;
- purifying the sample;
- reverse transcribing genetic material of the sample;
- performing PCR on the genetic material;
- and
- analyzing the results of the PCR.
Dependencies are inferred from the claim text.