Polymerase chain reaction container and process for producing the same
AssigneeMatsushita Electric Industrial Co., Ltd.
InventorsMasaya Nakatani, Tetsuo Yukimasa
Life of this patent
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Abstract
The present invention provides a kit employed for polymerase chain reaction. The kit has a cavity and a flow channel on a substrate. The flow channel is separated from the cavity by at least a barrier formed along the cavity. Such a structure allows the cavity to be filled with a sample solution even in minute quantities. At the same time, the structure can provide a sample solution with a rapid temperature-control. The structure can therefore contribute to accelerated polymerase chain reaction.
Claims31
A polymerase chain reaction kit comprising:
- a substrate on which a cavity, a flow channel for circulating a heating medium, and a barrier for separating the cavity and the flow channel are formed;
- and
- at least one cover plate of a plurality of cover plates for sealing at least one of the cavity and the flow channel.
A method of manufacturing a polymerase chain reaction kit comprising the steps of:
- a) forming a cavity and a flow channel for circulating a heating medium separated from the cavity on a surface of a substrate;
- b) attaching the substrate to at least one cover plate of a plurality of cover plates;
- c) connecting the cavity to a sample-injection inlet exposed to outside;
- and
- d) connecting the flow channel to a flow-in hole and a flow-out hole exposed to outside.
Dependencies are inferred from the claim text.