Systems for expression of heterologous proteins in m capsulatus

Recombinant vector comprising a first nucleotide sequence comprising the sequence of SEQ ID NO 3.

26b. Bacterial host cell according to claim 26, wherein the desired protein is expressed in the membrane.

26c. Bacterial host cell according to claim 26, wherein the desired protein is expressed on the surface of the outer membrane.

A method for producing a desired protein in a bacterial host cell, said method comprising transforming a bacterial host cell with a recombinant vector comprising a first nucleotide sequence comprising the sequence of SEQ ID NO 3, and said vector comprising a further nucleotide sequence encoding said protein, said further nucleotide sequence being operably linked in frame to said first nucleotide sequence, and culturing said transformed host cell in a suitable medium under conditions allowing expression of said protein.

Protein capable of being exposed on the surface of a methanotrophic bacterium, wherein the protein is encoded for by a nucleotide sequence comprising the sequence of SEQ ID No 3.

Fusion protein containing a protein or peptide sequence encoded for by a nucleotide sequence comprising the SEQ ID NO 3, and a further desired protein or peptide.

Nucleotide molecule wherein the molecule has a sequence which codes for a nucleotide sequence selected from the group comprising SEQ ID NOS 1-3 and 4-14.

Nucleotide molecule according to one of the preceding claims, wherein said molecule is incorporated and expressed in a methanotrophic bacterium.

Recombinant vector comprising a first nucleotide sequence selected from the group comprising SEQ ID NO 1-2 and SEQ iD NO 4-14.

A method for producing a desired protein in a bacterial host cell, said method comprising transforming a bacterial host cell with a recombinant vector comprising a first nucleotide sequence from the group comprising SEQ ID NO 1-2and SEQ ID NO 4-14, and said vector comprising a further nucleotide sequence encoding said protein, said further nucleotide sequence being operably linked in frame to said first nucleotide sequence, and culturing said transformed host cell in a suitable medium under conditions allowing expression of said protein.

Protein capable of being exposed on the surface of a methanotrophic bacterium, wherein the protein is encoded for by a nucleotide sequence selected from the group comprising SEQ ID NO 1-2 and SEQ ID NO 4-14.

Fusion protein containing a protein or peptide sequence encoded for by a nucleotide sequence selected from the group comprising SEQ ID NO 1 to SEQ ID NO 14, and a further desired protein or peptide.