Methods, compositions and kits for biomarker extraction
AssigneeMeso Scale Technologies, LLC.
InventorsJeff D. Debad, Cindy V. Ly
Life of this patent
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Abstract
The present invention is directed to methods for extracting markers from biological samples, and to systems, devices, kits and reagents for use in such methods. The invention is also to methods, kits, reagents and compositions for measuring a plurality of different organism types in a sample. One of the specific advantages of the present invention is the ability to simultaneously extract more than one microorganism or viral particle marker in one volume from a single sample containing a complex biological matrix.
Claims131
A method for measuring a plurality of different organisms in a sample comprising:
- (a) contacting said sample with an extraction reagent comprising nitrous acid, thereby forming an assay composition;
- and
- (b) measuring, in said assay composition, markers of said plurality of organisms so as to measure said plurality of different organisms.
A method for measuring a plurality of different organisms in an upper respiratory tract sample comprising:
- (a) contacting said upper respiratory tract sample with an extraction reagent comprising nitrous acid, thereby forming an assay composition;
- (b) incubating said assay composition under conditions suitable to extract a cell wall-associated antigen from a streptococcus bacterium;
- and
- (b) measuring, in said assay composition, said antigen and one or more additional markers including a marker of at least one virus.
A kit for measuring a plurality of different organism types in a sample comprising, in one or more containers:
- (a) an acid;
- (b) a nitrite salt;
- (c) a surfactant;
- (d) a first binding reagent that binds a first marker from a first of said plurality of different organism types and (e) a second binding reagent that binds a second marker from a second of said plurality of different organism types.
A kit for measuring a plurality of different organism types in a sample comprising, in one or more containers:
- (a) an acid;
- (b) a nitrite salt;
- (c) a surfactant;
- (d) a first antibody that binds a first marker that is a cell wall-associated antigen from a streptococcus bacterium and (e) a second antibody that binds a second marker from a virus.
A kit for measuring a plurality of different organism types in a sample comprising, in one or more containers:
- (a) an acid;
- (b) a nitrite salt;
- (c) a surfactant;
- (d) a first antibody that binds a first marker that is a group specific antigen from Streptococci Group A, B, F or G bacteria;
- (e) a second antibody that binds a second marker from influenza A;
- (f) a third antibody that binds a third marker from influenza B and (g) a fourth antibody that binds a fourth marker from respiratory syncytial virus (RSV).
A method for extracting two or more markers from a matrix comprising contacting a sample containing said matrix with an extraction reagent, wherein said extraction reagent comprises an oxidizing acid.
A method for extracting one or more markers from a matrix comprising contacting a sample containing said matrix with an extraction reagent, wherein said extraction reagent comprises an oxidizing acid and wherein said one or more markers includes a marker selected from the group consisting of protein, peptide, toxin, nucleic acid, lipid and combinations thereof.
A method for extracting one or more markers from a matrix comprising contacting a sample containing said matrix with an extraction reagent, wherein said extraction reagent comprises an oxidizing acid and wherein said one or more markers includes a viral marker, a fungal marker or combinations thereof.
A method for measuring two or more markers comprising:
- (a) contacting a sample containing one or more organisms with an extraction reagent comprising an oxidizing acid, thereby preparing an assay composition;
- and
- (b) measuring said two or more markers within said assay composition.
A method for measuring one or more markers comprising:
- (a) contacting a sample containing one or more organisms with an extraction reagent comprising an oxidizing acid, thereby preparing an assay composition;
- and
- (b) measuring said one or more markers in said assay composition, wherein at least one of said one or more markers includes a marker selected from the group consisting of protein, peptide, toxin, nucleic acid, and lipid.
A method for measuring one or more markers comprising:
- (a) contacting a sample containing one or more organisms with an extraction reagent comprising an oxidizing acid, thereby preparing an assay composition;
- and
- (b) measuring said one or more markers in said assay composition, wherein at least one of said one or more markers is a viral marker or a fungal marker.
A composition comprising two or more markers and an oxidizing acid.
A composition comprising one or more markers and an oxidizing acid, wherein at least one marker is a protein, peptide, toxin, nucleic acid, or lipid.
A composition comprising one or more markers and an oxidizing acid, wherein at least one marker is a viral or fungal marker.
A kit for extracting two or more markers from a sample for use in one or more assays comprising, in one or more containers:
- (a) an acid;
- (b) a nitrite salt;
- (c) a surfactant;
- and (d) at least one extraction component selected from the group consisting of:
- (i) a pH buffer/pH neutralizer;
- (ii) a sampler;
- (iii) preservatives;
- (iv) stabilizing agents;
- (v) extraction vessel;
- (vi) bleach;
- (vii) desiccants;
- (viii) capture moiety;
- and (ix) detection moiety.
A kit for extracting one or more markers from a sample for use in one or more assays comprising, in one or more containers:
- (a) an acid;
- (b) a nitrite salt;
- (c) a surfactant;
- and (d) at least one extraction component selected from the group consisting of:
- (i) a pH buffer/pH neutralizer;
- (ii) a sampler;
- (iii) preservatives;
- (iv) stabilizing agents;
- (v) extraction vessel;
- (vi) bleach;
- (vii) desiccants;
- (viii) capture moiety;
- and (ix) detection moiety;
- wherein at least one of said one or more markers is a viral or fungal marker.
A kit for extracting one or more markers from a sample for use in one or more assays comprising, in one or more containers:
- (a) an acid;
- (b) a nitrite salt;
- (c) a surfactant;
- and (d) at least one extraction component selected from the group consisting of:
- (i) a pH buffer/pH neutralizer;
- (ii) a sampler;
- (iii) preservatives;
- (iv) stabilizing agents;
- (v) extraction vessel;
- (vi) bleach;
- (vii) desiccants;
- (viii) capture moiety;
- and (ix) detection moiety;
- wherein at least one of said one or more markers is a protein, nucleic acid or lipid marker.
A clinical method for extracting markers of pathogenic microorganisms responsible for a disease of the upper respiratory tract comprising contacting a pharyngeal and/or nasal-pharyngeal swab or a nasal wash containing mucosa with an extraction reagent comprising an oxidizing acid and a detergent thereby extracting said markers from said mucosa.
A clinical method for extracting markers of pathogenic microorganisms responsible for a disease of the upper respiratory tract comprising contacting a pharyngeal and/or nasal-pharyngeal swab or a nasal wash containing mucosa with an extraction reagent comprising an oxidizing acid and a detergent thereby extracting said markers from said mucosa, and wherein one or more of said pathogenic microorganisms are present and at least one of said pathogenic microorganisms is a viral particle or a fungi.
A clinical method for extracting markers of pathogenic microorganisms responsible for a disease of the upper respiratory tract comprising contacting a pharyngeal and/or nasal-pharyngeal swab or a nasal wash containing mucosa with an extraction reagent comprising an oxidizing acid and a detergent thereby extracting said markers from said mucosa, and wherein two or more said pathogenic microorganisms are present and at least one of said pathogenic microorganisms is a viral particle or a fungi.
Dependencies are inferred from the claim text.