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US 2004/0175693 A1Published application
Nucleic acid biosensors
AssigneeThe Board of Trustees of the University of Illinois
InventorsYi Lu, Juewen Liu
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Abstract
Sensors comprising aptazymes capable of detecting the presence and concentration of effectors, as well as methods of using such sensors, are disclosed.
Claims29
A sensor system for detecting an effector, comprising:
- (a) a nucleic acid enzyme, comprising an aptamer comprising a binding site for the effector;
- (b) a substrate for the nucleic acid enzyme, comprising a first polynucleotide;
- and
- (c) particles comprising a second polynucleotide at least partially complementary to the substrate.
1 more element
A sensor system for detecting an effector, comprising:
- (a) a nucleic acid enzyme, comprising an aptamer comprising a binding site for the effector;
- (b) a substrate for the nucleic acid enzyme, comprising a first polynucleotide;
- (c) gold particles comprising a second polynucleotide at least partially complementary to the substrate;
- and
- (d) Mg(II);
- wherein the nucleic acid enzyme comprises DNA.
3 more elements
A method of detecting an effector, comprising mixing together a set of ingredients comprising:
- a sample;
- a nucleic acid enzyme, comprising an aptamer comprising a binding site for the effector;
- a substrate for the nucleic acid enzyme, comprising a first polynucleotide;
- and
- particles comprising a second polynucleotide that is at least partially complementary to the substrate.
2 more elements
A kit for detecting an effector, comprising
- (a) a nucleic acid enzyme, comprising an effector binding site comprising an aptamer;
- (b) a substrate for the nucleic acid enzyme, comprising a first polynucleotide;
- and
- (c) particles comprising a second polynucleotide at least partially complementary to the substrate.
1 more element
Dependencies are inferred from the claim text.