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AssigneeThe Wistar Institute of Anatomy and Biology
InventorsMeenhard Herlyn, Jiri Kalabis
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An organotypic culture comprises an artificial stroma overlayed with epithelial cells isolated from a human colon or intestine. The stroma comprises a mixture of collagen and human fibroblasts isolated from a human colon or intestine. The culture contains a factor that binds the IGF-1 receptor, a factor that binds the EGF receptor, and a factor that binds the LIF receptor. These factors may be added exogenously to the culture via medium or may be expressed by various recombinantly engineered cell types in the culture. The organotypic culture can result in growth that is in situ-like or emphasizes other physiological or morphological states, depending on the balance of factors in the growth media. The organotypic culture may be used in methods for screening of therapeutic, carcinogenic, or growth enhancement factors, or for treating intestinal injuries by applying to the site of an injury the intact culture or the components thereof.
An organotypic culture comprising an artificial stroma comprising a mixture of collagen and human fibroblasts isolated from a human colon or intestine, said stroma overlayed with epithelial cells isolated from a human colon or intestine, wherein present in said culture are at least one growth factor that binds the insulin growth factor-1 (IGF-1) receptor, at least one growth factor that binds the epidermal growth factor (EGF) receptor, and at least one growth factor that binds the leukemia inhibitory factor (LIF) receptor.
The culture according to claim 26 wherein said vector is an adenovirus.
An organotypic culture comprising an artificial stroma comprising a mixture of collagen and human fibroblasts isolated from a human colon or intestine, said stroma overlayed with epithelial cells isolated from a human colon or intestine, wherein present in said culture are at least one growth factor that binds the IGF—I receptor, at least one growth factor that binds the EGF receptor, at least one growth factor that binds the LIF receptor, at least one growth factor that binds the ET-3 receptor, at least one growth factor that binds the HGF receptor, at least one growth factor that binds the SCF receptor, and at least one growth factor that binds the AMF receptor and a combination of two or more said factors.
The method according to claim 37, wherein said growth factor that binds the IGF-1 receptor is selected from the group consisting of insulin and IGF-1.
A method for enhancing epithelial cell repair at an in vivo site of intestinal or colonic injury comprising:
Dependencies are inferred from the claim text.