Microscale affinity purification system
OwnerAlexei Belenky, Yuriy Dunayevskiy +1inventors
InventorsAlexei Belenky, Yuriy Dunayevskiy, Dallas E. Hughes
Life of this patent
Estimating the term…
Abstract
A microscale affinity purification system has a plurality of capillary channels (100) which begin and end in common compartments (110, 120). An introduction cross-capillary channel (130) runs across the capillary channels (100) in a serpentine pattern (136, 138) near one end and a collection channel (140) similarly crosses the capillaries (100) in a serpentine pattern (148, 150) near the other end. A target molecule is introduced from a reservoir (132) which binds to a desired strong ligand in a sample in the capillaries (100). The target-strong ligand comlex migrates through the capillaries (100), is detected at a detector (146), and <collected in a collection reservoir (144).
Claims37
A microscale affinity purification system comprising:
- a substrate;
- a plurality of longitudinally extending capillary channels formed in the substrate, the capillary channels connected at one end to a first common source and connected at an opposite end to a second common source;
- a first analyte movement subsystem operative to move analyte along the plurality of capillary channels from the first common source to the second common source or from the second common source to the first common source;
- an introduction cross-capillary channel formed in the substrate and extending across the plurality of capillary channels near the first common source, the introduction cross-capillary channel comprising transverse portions connecting adjacent ones of the capillary channels and coinciding portions that coincide with portions of the capillary channels to impart a generally serpentine configuration to the introduction cross-capillary channel;
- a collection cross-capillary channel formed in the substrate and extending across the plurality of capillary channels near the second common source, the collection cross-capillary channel comprising transverse portions connecting adjacent ones of the capillary channels and coinciding portions that coincide with portions of the capillary channels to impart a generally serpentine configuration to the collection cross-capillary channel;
- a second analyte movement subsystem operative to move analyte along the introduction cross-capillary channel;
- and
- a third analyte movement subsystem operative to move analyte along the collection cross-capillary channel.
A microscale affinity purification system comprising:
- a substrate;
- a longitudinally extending capillary channel formed in the substrate, the capillary channel connected at one end to a first source of buffer and connected at an opposite end to a second source of buffer;
- a first analyte movement subsystem operative to move analyte along the capillary channel from a first reservoir to a second reservoir or from the second reservoir to the first reservoir;
- an introduction cross-capillary channel formed in the substrate and extending across the capillary channel near the first reservoir, at least a portion of the introduction cross-capillary channel comprising a coinciding portion that coincides with a portion of the capillary channel;
- a target source in analyte communication with the introduction cross-capillary channel;
- a target reservoir in analyte communication with the capillary channel to receive excess target;
- a collection cross-capillary channel formed in the substrate and extending across the capillary channel near the second reservoir, at least a portion of the collection cross-capillary channel comprising a coinciding portion that coincides with a portion of the capillary channel;
- a buffer source in analyte communication with the collection cross-capillary channel;
- a collection reservoir in analyte communication with the collection channel to receive target/ligand complex;
- a second analyte movement subsystem operative to move analyte along the introduction cross-capillary channel;
- and
- a third analyte movement subsystem operative to move analyte along the collection cross-capillary channel.
Dependencies are inferred from the claim text.