Method for detecting the impacts of interfering effects on experimental data
OwnerChristian Eggeling, Kaupo Palo +3inventors
InventorsChristian Eggeling, Kaupo Palo, Nicolas Fay, Maciej Hoffman-Wecker, Pierre Ilouga
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Abstract
The invention provides a method for identifying the impacts of interfering effects on experimental data. In particular, a method is described for identifying the impacts of unwanted auto-fluorescence, fluorescence quenching, and deterioration of a fluorescent sample under study on the collected experimental data. The data are analyzed whether or not said data fulfill certain criteria with respect to a threshold which is indicative for said interfering effect.
Claims44
A method for detecting the impacts of interfering effects on data, such as experimental data, comprising the steps of:
- (i) providing the data,
- (ii) determining values of one or a plurality of identification parameters from said data,
- (iii) creating a histogram or distribution of the values of the identification parameters,
- (iv) determining one or a plurality of thresholds for the values of identification parameters from said histogram or distribution, which thresholds are indicative for the interfering effects,
- (v) analyzing the values of one or a plurality of identification parameters whether or not these values fulfill one or a plurality of criteria with respect to the thresholds, and
- (vi) determining those data which are influenced and/or those data which are not affected by the interfering effects.
A method for detecting the impacts of auto-fluorescence and/or fluorescence quenching on experimental data resulting from fluorescence experiments comprising the steps of:
- (i) providing the experimental data comprising a plurality of data sets,
- (ii) determining values of one or a plurality of identification parameters from said data sets,
- (iii) creating a histogram or distribution of the values of the identification parameters,
- (iv) determining one or a plurality of first thresholds for the values of identification parameters from said histogram or distribution, which first thresholds are indicative for auto-fluorescence, and/or determining one or a plurality of second thresholds for the values of identification parameters from said histogram or distribution, which second thresholds are indicative for fluorescence quenching,
- (v) analyzing the values of one or a plurality of identification parameters whether or not these fulfill one or a plurality of criteria with respect to the thresholds, and
- (vii) determining those data sets which are influenced and/or those data sets which are not affected by auto-fluorescence and/or fluorescence quenching.
A method for detecting false positive and/or false negative results in data, in particular experimental data resulting from screening of potentially pharmaceutical active compounds, comprising the steps of:
- (i) providing the data,
- (ii) determining values of one or a plurality of identification parameters from said data,
- (iii) creating a histogram or distribution of the values of the identification parameters,
- (iv) determining one or a plurality of first thresholds for the values of identification parameters from said histogram or distribution, which first thresholds are indicative for a false-positive result, and/or determining one or a plurality of second thresholds for the values of identification parameters from said histogram or distribution, which second thresholds are indicative for a false-negative result,
- (v) analyzing the values of one or a plurality of identification parameters whether or not these fulfill one or a plurality of criteria with respect to the thresholds, and
- (vi) determining those data which represent a false-positive result and/or those data which represent a false-negative result.
A system for detecting the impacts of interfering effects on experimental data resulting from optical experiments, said system comprising:
- (i) means for supporting one or a plurality of samples in an inspection station (2),
- (ii) one or a plurality of photosensitive detectors (9, 10) which are positioned relative to the inspection station (2) so that electromagnetic radiation emitted from the samples impinges on the detectors (9, 10), and a
- (iii) signal processing unit (11) comprising
- means for addressing the photosensitive detectors (9, 10) to generate experimental data,
- means for determining values of one or a plurality of identification parameters from said data,
- means for storing the values in such a manner that preferably all the values which relate to any one of the samples are linked,
- means for creating a histogram or distribution of the values of the identification parameters,
- means for determining one or a plurality of thresholds for the values of identification parameters from said histogram or distribution, which thresholds are indicative for the interfering effects,
- means for analyzing the values of one or a plurality of identification parameters whether or not these fulfill one or a plurality of criteria with respect to the thresholds, and
- means for supplying as output information those data which are influenced and/or those data which are not affected by the interfering effects.
A signal processing unit (11) for processing experimental data which may be deteriorated by interfering effects comprising
- means for storing experimental data,
- means for determining values of one or a plurality of identification parameters from said data,
- means for storing the values in such a manner that preferably all the values which relate to any one of the samples are linked,
- means for creating a histogram or distribution of the values of the identification parameters,
- means for determining one or a plurality of thresholds for the values of identification parameters from said histogram or distribution, which thresholds are indicative for the interfering effects,
- means for analyzing the values of one or a plurality of identification parameters whether or not these fulfill one or a plurality of criteria with respect to the thresholds, and
- means for supplying as output information those data which are influenced and/or those data which are not affected by the interfering effects.
Dependencies are inferred from the claim text.