Modified proteinase inhibitors
OwnerHoward John Atkinson, Michael John McPherson +1inventors
InventorsHoward John Atkinson, Michael John McPherson, Peter Edward Urwin
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Abstract
Modified proteinase inhibitors having enhanced inhibition efficacy when compared to the naturally occurring counterparts. Also provided are polynucleotide molecules encoding the modified proteinase inhibitors, vectors and other recombinant nucleic acid constructs, transgenic cells, and transgenic organisms that comprise the polynucleotide molecules. Further disclosed are methods for producing the modified proteinase inhibitors, and methods and compositions for increasing the resistance of a cell or an organism to proteolytic damages, particularly the damages caused by a pest or a pathogen.
Claims28
An isolated polynucleotide molecule comprising a sequence that encodes a polypeptide sequence, wherein said polypeptide sequence is encoded by CYT1-ORYSA-D86 of FIG. 1, and has increased proteinase inhibiting activity when compared to a naturally-occurring cystatin.
An isolated polynucleotide molecule encoding a modified cystatin which has increased proteinase inhibiting activity when compared to its naturally-occurring counterpart, and which comprises at least a deletion of an amino acid residue corresponding to aspartic acid at position 86 of oryzacystatin.
A modified cystatin which has increased proteinase inhibiting activity when compared to its naturally-occurring counterpart, and comprises at least a deletion of an amino acid residue corresponding to aspartic acid at position 86 of oryzacystatin I.
A hybrid proteinase inhibitor comprises at least a partial sequence of an animal cystatin and at least a partial sequence of a piant cystatin linked together, wherein the hybrid proteinase inhibitor has an increased proteinase inhibitor activity when compared to a naturally occurring counterpart of said plant cystatin.
Dependencies are inferred from the claim text.