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OwnerJohn Mundy, Anders Bogh Jensen +3inventors
InventorsJohn Mundy, Anders Bogh Jensen, Morten Petersen, Henrik Naested, Peter Brodersen
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Uses of MAPK4, a member of the mitogen-activated protein (MAP) kinase family, are provided herein, based on the discovery that the MAPK4 negatively regulates the expression of genes associated with disease (e.g. PR-genes) and wound responses in plants such that the loss of MAPK4 function leads to their derepression. Methods are disclosed for controlling the growth of a plant and/or the expression of at least one wounding or pathogen response gene in said plant, the method comprising altering in the plant the level of the gene product of a MAPK4 gene. Furthermore, transgenic plants transformed with an MAPK4 construct and having enhanced wound and/or disease resistance are disclosed.
A method of controlling the growth of a plant and/or the expression of at least one wounding or pathogen response gene in said plant, the method comprising altering in the plant the level of the gene product of a MAPK4 gene.
A transgenic plant having enhanced wound and/or disease resistance, said plant comprising an antisense MAPK4 construct, wherein said construct leads to an increase in the expression of wounding and/or pathogen responsive genes.
A transgenic plant having enhanced wound and/or disease resistance, said plant comprising a constitutively active form of MAPK4.
A transgenic plant having enhanced wound and/or disease resistance, said plant comprising a catalytically inactive MAPK4 construct, wherein said construct leads to an increase in the expression of wounding and/or pathogen responsive genes.
A transgenic plant having enhanced wound and/or disease resistance, said plant comprising a mutation in the MAPK4 gene which results in a loss of function of said gene, wherein said mutation leads to an increase in the expression of wounding and/or pathogen responsive genes.
A recombinant DNA construct comprising the coding region of MAPK4 gene operably linked in an antisense orientation to an appropriate promoter.
A method of screening a plant population for plants carrying an insertion element within the MAPK4 gene whereby the gene is functionally inactivated, the method comprising the steps of
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