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AssigneeMetabolix, Inc.
InventorsLara Madison, Gjalt W. Huisman, Oliver P. Peoples
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Methods for engineering transgenic organisms that synthesize polyhydroxyalkanoates (PHAs) containing 3-hydroxyhexanoate as comonomer have been developed. These processes are based on genetically engineered bacteria such as Escherichia coli or in plant crops as production systems which include PHA biosynthetic genes from PHA producers. In a preferred embodiment of the method, additional genes are introduced in wild type or transgenic polyhydroxybutyrate (PHB) producers, thereby creating new strains that synthesize 3HH monomers which are incorporated into PHAs. The 3HH monomer preferably is derived in microbial systems using butanol or butyrate as feedstocks, which are precursors of 3-hydroxyhexanoyl-CoA. Pathways for in vivo production of butyrol-CoA specifically encompassing butyryl-CoA dehydrogenase activity are provided.
A method for the biological production of polyhydroxyalkanoates containing 3-hydroxyhexanoate comprising synthesizing the polyhydroxyalkanoate in a transgenic organism having at least one transgene encoding an enzyme selected from the group consisting of PHB polymerase, PHA polymerase, β-ketothiolase, 13-ketoacyl-CoA reductase, D-specific enoyl-CoA hydratase, crotonase, butyryl-CoA dehydrogenase, and 3-hydroxybutyryl-CoA dehydrogenase integrated into the chromosome.
The method of 9 wherein the hydratase gene is isolated from a bacteria selected from the group consisting of R. eutropha, Klebsiella aerogenes, P. putida, and Aeromonas caviae.
A method for producing polyhydroxybutyrate-co-3hydroxyhexanoate comprising feeding an organism
A method for producing 3-hydroxyhexanoate copolymers comprising
A method for producing 3-hydroxyhexanoate copolymers comprising
A polyhydroxybutyrate-co-3-hydroxyhexanoate produced in a genetically engineered Escherichia coli K12.
Dependencies are inferred from the claim text.