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OwnerFrederick M. Ausubel, Brian J. Staskawicz +9inventors
InventorsFrederick M. Ausubel, Brian J. Staskawicz, Andrew F. Bent, Douglas Dahlbeck, Fumiaki Katagiri, Barbara N. Kunkel, Michael Nicholas Mindrinos, Guo-Liang Yu, Barbara Baker, Jeffrey Ellis, John Salmeron
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Disclosed is substantially pure DNA encoding an Arabidopsis thaliana Rps2 polypeptide; substantially pure Rps2 polypeptide; and methods of using such DNA to express the Rps2 polypeptide in plant cells and whole plants to provide, in transgenic plants, disease resistance to pathogens. Also disclosed are conserved regions characteristic of the RPS family and primers and probes for the identification and isolation of additional RPS disease-resistance genes.
Substantially pure DNA encoding an Rps polypeptide.
Substantially pure DNA having the sequence of FIG. 2, or degenerate variants thereof, and encoding the amino acid sequence of open reading frame “a” of FIG. 2.
Substantially pure DNA having about 50% or greater sequence identity to the DNA sequence of FIG. 2.
A method of producing an Rps2 polypeptide comprising:
A method of isolating a disease resistance gene or portion thereof in plants having sequence identity to RPS2, said method comprising:
A substantially pure Rps 2 polypeptide.
A vector comprising the DNA of the avrRpt2 gene operably linked to regulatory sequences wherein said regulatory sequences comprise a promoter.
A substantially pure oligonucleotide comprising the sequence:
A substantially pure oligonucleotide comprising the sequence:
A substantially pure oligonucleotide comprising the sequence:
A substantially pure oligonucleotide comprising the sequence:
A substantially pure oligonucleotide comprising the sequence:
A substantially pure oligonucleotide comprising the sequence:
A substantially pure oligonucleotide comprising the sequence:
A substantially pure oligonucleotide comprising the sequence:
A substantially pure oligonucleotide comprising the sequence:
A recombinant plant gene comprising the DNA sequence:
A substantially pure plant polypeptide comprising the amino acid sequence:
A substantially pure plant polypeptide comprising the amino acid sequence:
A substantially pure plant polypeptide comprising the amino acid sequence:
A substantially pure plant polypeptide comprising the amino acid sequence:
A substantially pure plant polypeptide comprising the amino acid sequence:
A method of isolating a disease-resistance gene or fragment thereof from a plant cell, comprising:
A method of identifying a plant disease-resistance gene in a plant cell, comprising:
A recombinant plant polypeptide capable of conferring disease-resistance wherein said plant polypeptide comprises a P-loop domain or nucleotide binding site domain.
A recombinant plant polypeptide capable of conferring disease-resistance wherein said plant polypeptide contains a leucine-rich repeating domain.
A plant disease-resistance gene isolated according to the method comprising:
A plant disease-resistance gene isolated according to the method comprising:
A method of identifying a plant disease-resistance gene comprising:
A plant disease-resistance gene isolated according to the method comprising:
A purified antibody which binds specifically to an rps family protein.
A DNA sequence substantially identical to the DNA sequence shown in FIG. 12.
A substantially pure polypeptide having a sequence substantially identical to a Prf amino acid sequence shown in FIG. 5(A or B).
Dependencies are inferred from the claim text.