Method for producing transgenic monocotyledonous plants
OwnerTheresa C. Wilkinson, Henry T. Wilkinson +1inventors
InventorsTheresa C. Wilkinson, Henry T. Wilkinson, Richard E. Wagner
Life of this patent
Estimating the term…
Abstract
Methods for transforming monocot plants are provided. The method comprise the steps of excising an embryo from a seed of the plant, allowing the embryo to develop to a stage highly receptive for transformation, transforming the developing embryo, and developing the embryo into a plantlet, wherein the transformation occurs without passing through a callus phase.
Claims16
A method for transforming a monocot plant, said method comprising the steps of:
- excising an embryo from a seed of the plant,
- placing the excised embryo on media not amended with growth hormones,
- allowing the embryo to develop to a stage highly receptive for transformation,
- transforming the developing embryo,
- transferring the transformed embryo to a hormone-free selective media that supports growth of the embryo, and
- developing the embryo into a plantlet.
A method for transforming a monocot plant, said method comprising the steps of:
- excising an embryo from a seed of the plant,
- allowing the embryo to develop to a stage highly receptive for transformation,
- transforming the developing embryo, and
- developing the embryo into a plantlet,
- wherein the allowing, transforming, and developing steps all occur without passing through a callus phase.
A method for transforming a monocot plant, said method comprising the steps of:
- excising an embryo from a seed of the plant,
- placing the excised embryo on media essentially free of growth hormones,
- allowing the embryo to develop to a stage highly receptive for transformation,
- transforming the developing embryo,
- transferring the transformed embryo to a selective medium that supports growth of the embryo essentially free of growth hormones, and
- developing the embryo into a plantlet.
Dependencies are inferred from the claim text.