Maize yellow stripe1 and related genes
OwnerElsbeth L. Walker, Stephen Dellaportainventors
Life of this patent
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Abstract
The maize yellow stripe1 gene encodes a transmembrane protein which mediates the uptake of chelated iron from soil. The gene is shown here to be upregulated, and protein production increased, under conditions of low iron bioavailability. The protein is also shown to be able to transport other metals as well, such as copper. The present invention also provides a family of Arabidopsis genes and gene products related to ys1 termed here as ys11-8. The invention provides the genes and their gene products as well as methods to create and use transgenic plants for the increased uptake of iron in nutritional crops as well as for the bioremediation of soil.
Claims28
An isolated nucleic acid molecule selected from the group consisting of:
- (a) an isolated nucleic acid molecule that encodes the amino acid sequence of SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 16 or 18;
- (b) an isolated nucleic acid molecule that encodes a fragment of at least 6 amino acids of SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 16 or 18;
- (c) an isolated nucleic acid molecule which hybridizes to the complement of a nucleic acid molecule comprising SEQ ID NO:
- 1, 3, 5, 7, 9, 11, 13, 15 or 17;
- (d) an isolated nucleic acid molecule which hybridizes to the complement of a nucleic acid molecule that encodes the amino acid sequence of SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 16 or 18 and which encodes a YS1 or an Arabidopsis YSL protein;
- and (e) an isolated nucleic acid molecule that encodes a protein that exhibits at least about 79% amino acid sequence identity to SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 16 or 18.
An isolated polypeptide or protein selected from the group consisting of:
- (a) an isolated polypeptide comprising the amino acid sequence of SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 16 or 18;
- (b) an isolated polypeptide comprising a fragment of at least 6 amino acids of SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 16 or 18;
- (c) an isolated polypeptide comprising conservative amino acid substitutions of SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 16 or 18;
- (d) naturally occurring amino acid sequence variants of SEQ ID NO:
- 2;
- and (e) an isolated polypeptide exhibiting at least about 79% amino acid sequence identity with SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 16 or 18.
Dependencies are inferred from the claim text.