Glyphosate tolerant sugar beet
AssigneeKws Saat AG
InventorsJosef Kraus, Elke Sauerbrey, Reinhard Nehls, Andreas Loock, Rudolf Jansen
Life of this patent
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Abstract
The invention relates to glyphosate tolerant sugar beet plants, plant material and seeds. It is the object of the invention to provide a transgenic sugar beet plant event that shows a high level of tolerance against glyphosate, but is not impaired in other important agronomic properties such as growth, yield, quality, pathogen resistance etc.
Claims14
Glyphosate tolerant sugar beet plant, characterized in that a) the sugar beet plant is obtained from seed deposited with the NCIMB and having the accession number NCIMB 41158 or NCIMB 41159, and/or b) a DNA fragment of between 630-700 bp, preferably 664 bp, can be amplified from the genomic DNA of said sugar beet plant, parts or seeds thereof, using polymerase chain reaction with a first primer having the nucleotide sequence of SEQ ID NO:
- 1 and a second primer having the nucleotide sequence of SEQ ID NO:
- 2, and/or c) a DNA fragment of 3500-3900, preferably 3706 bp, can be amplified from the genomic DNA of said sugar beet plant, parts or seeds thereof, using polymerase chain reaction with a first primer having the nucleotide sequence of SEQ ID NO:
- 3 and a second primer having the nucleotide sequence of SEQ ID NO:
- 4, and/or d) a DNA fragment of 270-300 bp, preferably 288 bp, can be amplified from the genomic DNA of said sugar beet plant, parts or seeds thereof, using polymerase chain reaction with a first primer having the nucleotide sequence of SEQ ID NO:
- 7 and a second primer having the nucleotide sequence of SEQ ID NO:
- 8, and/or e) a DNA fragment of 710-790 bp, preferably 751 bp, can be amplified from the genomic DNA of said sugar beet plant, parts or seeds thereof, using polymerase chain reaction with a first primer having the nucleotide sequence of SEQ ID NO:
- 9 and a second primer having the nucleotide sequence of SEQ ID NO:
- 10, and/or f) a DNA fragment of 990-1100 bp, preferably 1042 bp, can be amplified from the genomic DNA of said sugar beet plant, parts or seeds thereof, using polymerase chain reaction with a first primer having the nucleotide sequence of SEQ ID NO:
- 14 and a second primer having the nucleotide sequence of SEQ ID NO:
- 16.
Seed deposited with the NCIMB and having the accession number NCIMB 41158 or NCIMB 41159.
Method for identification of a glyphosate tolerant sugar beet plant, characterized in that the method comprises the step(s) of a) amplifying a DNA fragment of between 630-700 bp, preferably 664 bp, from the genomic DNA of said sugar beet plant, parts or seeds thereof, using polymerase chain reaction with a first primer having the nucleotide sequence of SEQ ID NO:
- 1 and a second primer having the nucleotide sequence of SEQ ID NO:
- 2, and/or b) amplifying a DNA fragment of 3500-3900, preferably 3706 bp, from the genomic DNA of said sugar beet plant, parts or seeds thereof, using polymerase chain reaction with a first primer having the nucleotide sequence of SEQ ID NO:
- 3 and a second primer having the nucleotide sequence of SEQ ID NO:
- 4, and/or c) amplifying a DNA fragment of 270-300 bp, preferably 288 bp, from the genomic DNA of said sugar beet plant, parts or seeds thereof, using polymerase chain reaction with a first primer having the nucleotide sequence of SEQ ID NO:
- 7 and a second primer having the nucleotide sequence of SEQ ID NO:
- 8, and/or d) amplifying a DNA fragment of 710-790 bp, preferably 751 bp, from the genomic DNA of said sugar beet plant, parts or seeds thereof, using polymerase chain reaction with a first primer having the nucleotide sequence of SEQ ID NO:
- 9 and a second primer having the nucleotide sequence of SEQ ID NO:
- 10, and/or e) amplifying a DNA fragment of 990-1100 bp, preferably 1042 bp, from the genomic DNA of said sugar beet plant, parts or seeds thereof, using polymerase chain reaction with a first primer having the nucleotide sequence of SEQ ID NO:
- 14 and a second primer having the nucleotide sequence of SEQ ID NO:
- 16.
Test kit for identifying a transgenic glyphosate tolerant sugar beet plant, its cells, tissue or parts, comprising at least one primer pair with a first and a second primer for polymerase chain reaction, the first primer recognizing a sequence within the foreign DNA incorporated into the genome of said plant, and the second primer recognizing a sequence within the 3′ or 5′ flanking regions of said DNA, characterized in that a) the first primer has the nucleotide sequence of SEQ ID NO:
- 1 and the second primer has the nucleotide sequence of SEQ ID NO:
- 2, and/or b) the first primer has the nucleotide sequence of SEQ ID NO:
- 7 and the second primer has the nucleotide sequence of SEQ ID NO:
- 8, and/or c) the first primer has the nucleotide sequence of SEQ ID NO:
- 9 and the second primer has the nucleotide sequence of SEQ ID NO:
- 10, and/or d) the first primer has the nucleotide sequence of SEQ ID NO:
- 14 and the second primer has the nucleotide sequence of SEQ ID NO:
- 16.
Dependencies are inferred from the claim text.