Administration of transposon-based vectors to reproductive organs
OwnerRichard K. Cooper, William C. Fioretti +1inventors
InventorsRichard K. Cooper, William C. Fioretti, Gary G. Cadd
Life of this patent
Estimating the term…
Abstract
Methods and compositions for the administration of transposon-based vectors to the reproductive organs of animals and the creation of transgenic animals. Preferred methods involve administration of the transposon-based vectors to the lumen of the oviduct of an avian, expression of a vector derived transgene in the avian, and deposition of the resultant polypeptide in an egg. This invention allows for large amounts of protein to be deposited in the egg.
Claims44
A method of producing a transgenic animal comprising, administering to an oviduct or an ovary of an animal a composition comprising a transposon-based vector.
A method of increasing expression of a polynucleotide sequence in an avian comprising, administering to the avian a composition comprising the polynucleotide sequence operably-linked to an avian optimized polyA sequence.
A method of producing proteins, polypeptides or peptides comprising:
- a) administering to an oviduct of an egg-laying animal a composition comprising a transposon-based vector, wherein the transposon-based vector comprises a transposase gene operably linked to a first promoter, the transposase gene encoding for a transposase, and one or more genes of interest operably-linked to one or more additional promoters;
- and,
- b) permitting the one or more genes of interest to be expressed into a protein, a polypeptide or a peptide.
A method of producing a transgenic mammal comprising, administering to an intramammary duct system of a mammal a composition comprising a transposon-based vector.
A method of producing proteins, polypeptides or peptides comprising:
- a) administering to an intramammary duct system of a mammal a composition comprising a transposon-based vector, wherein transposon-based vector comprises a transposase gene operably linked to a first promoter, the transposase gene encoding for a transposase, and one or more genes of interest operably-linked to one or more additional promoters;
- and,
- b) permitting the one or more genes of interest to be expressed into a protein, a polypeptide or a peptide.
Dependencies are inferred from the claim text.