A Lac operator-repressor system
OwnerHeidi Jean Scrable, Carolyn Anne Cronininventors
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Abstract
The present invention relates to a system that allows the specific regulation of a gene in a eukaryotic cell. The system comprises a novel repressor gene construct (shown in FIG. 1), wherein the construct comprises a promoter (stippled box) operably linked to a rabbit β-globin intron2/exon3 sequence (open box) which is operably linked to a modified lac repressor coding region which is operably linked to rabbit β-globin 3′ untranslated sequences (solid bar). The modified lac repressor coding region is made up of segments that are identical to the wild type bacterial sequence (crosshatched box), and segments that have been reencoded to use mammalian codons (striped box).
Claims25
A nucleic acid sequence comprising the sequence of SEQ ID NO:
- 1.
A method of regulating the expression of an endogenous gene in vivo said method comprising the steps of
- providing a transgenic animal that comprises an operator sequence inserted into an endogenous gene;
- and
- introducing a repressor encoding nucleic acid sequence, comprising a eukaryotic promoter operably linked to the sequence of SEQ ID NO:
- 4, into that transgenic animal or its progeny.
An operator targeting construct comprising
- an operator sequence;
- two direct repeats of a site specific recombinase target sequence;
- and
- a reporter gene construct, said reporter gene construct comprising a reporter gene and a 3′ splice acceptor site located upstream from said reporter gene, wherein the operator is operably linked to the reporter gene construct and said reporter gene construct is flanked by direct repeats of the site-specific recombinase target sequence.
Dependencies are inferred from the claim text.