Hollow fiber membrane sample preparation devices

A sample purification and enrichment method comprising:

• simultaneously enriching and cleaning up an analyte of interest by extracting the analyte of interest from a donor sample into a static acceptor liquid through a liquid extraction membrane formed in a wall of a porous hollow fiber situated in a well of a multi-well plate, the hollow fiber enclosing the acceptor liquid;
• and
• transferring the analyte of interest from the hollow fiber to an analysis device.

A sample purification and enrichment method comprising:

• simultaneously enriching and cleaning up an analyte of interest by extracting the analyte of interest from a donor sample into a static acceptor liquid through a liquid extraction membrane formed in a porous extraction disk situated in a well of a multi-well plate;
• and
• transferring the analyte of interest from the well to an analysis device.

A hollow-fiber membrane sample preparation multi-well plate for enriching and cleaning up samples, comprising:

• a plurality of wells for holding a corresponding plurality of donor samples, each donor sample comprising an analyte of interest;
• and
• a plurality of porous hollow fibers situated in the corresponding plurality of wells, each hollow fiber being situated in one of the wells, each hollow fiber including a liquid extraction membrane enclosing an internal cavity of the hollow fiber, for holding a static acceptor liquid within each hollow fiber to receive the analyte of interest through the liquid extraction membrane into the acceptor liquid.

A hollow-fiber membrane sample preparation plate for enriching and cleaning up samples, comprising:

• a planar top plate having a plurality of access apertures defined therethrough, a spacing of the access apertures being chosen such that each access aperture can be aligned to a well of a sample-holding well block;
• and
• a plurality of porous hollow fibers hanging from the planar top plate such that each access aperture provides access to an internal cavity defined within one of the fibers, each hollow fiber including a liquid extraction membrane enclosing the internal cavity, for holding a static acceptor liquid within each hollow fiber to receive an analyte of interest from a sample held in the well through the liquid extraction membrane into the acceptor liquid.

A sample preparation kit comprising:

• a multi well plate comprising a plurality of wells for holding a corresponding plurality of donor samples each comprising an analyte of interest;
• and
• a top plate comprising plurality of porous hollow fibers spaced apart so as to be inserted in the corresponding plurality of wells, each hollow fiber including a liquid extraction membrane for transferring the analyte of interest from the donor solvent to an acceptor liquid through the liquid extraction membrane.

A hollow-fiber membrane sample preparation multi-well plate for enriching and cleaning up samples, comprising:

• well means comprising a plurality of wells for holding a corresponding plurality of donor samples, each donor sample comprising an analyte of interest;
• and
• hollow fiber support means for holding a plurality of porous hollow fibers in the well means, each hollow fiber being situated in one of the wells, each hollow fiber including a liquid extraction membrane enclosing an internal cavity of the hollow fiber, for holding a static acceptor liquid within each hollow fiber to receive the analyte of interest through the liquid extraction membrane into the acceptor liquid.