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US 2004/0171156 A1Published application
Recombinational cloning using nucleic acids having recombination sites
AssigneeInvitrogen Corporation
InventorsJames L. Hartley, Michael A. Brasch, Gary F. Temple, Donna K. Fox
Life of this patent
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Abstract
Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
Claims18
An in vitro method of cloning an amplification product comprising:
- (a) obtaining an amplification product comprising a first recombination site and a second recombination site which do not recombine with each other;
- and
- (b) combining said amplification product in vitro with a vector comprising a third recombination site and a fourth recombination site which do not recombine with each other, under conditions such that recombination occurs between said first and third and said second and fourth recombination sites, thereby producing a product vector.
Dependencies are inferred from the claim text.