Stem cell
OwnerPeter Andrews, James Walsh +1inventors
InventorsPeter Andrews, James Walsh, Paul Gokhale
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Abstract
There is provided a method to modulate the differentiation state of embryonic stem cells in culture by the providing ligands which bind receptors in the Notch and Wnt pathways.
Claims26
A method to modulate the differentiation of an embryonic stem cell comprising:
- i) providing a culture of embryonic stem cells;
- ii) providing at least one ligand, or the active binding fragment thereof, capable of binding its cognate receptor polypeptide expressed by said embryonic stem cell;
- iii) forming a culture comprising embryonic stem cells and said ligand;
- and
- iv) growing said cell culture.
A method for modulating the differentiation of embryonic stem cells comprising:
- i) providing a cell transfected with a nucleic acid molecule selected from the group consisting of.
- a) a nucleic acid molecule as represented in FIGS. 2, 4, 5, 7, 10, 12, 14, 16, 18.
- b) a nucleic acid molecule which hybridises to the nucleic acid in (ii) and which encodes a ligand capable of modulating embryonic stem cell differentiation;
- and
- c) nucleic acid molecules which are degenerate as a result of the genetic code to the sequences defined in (a) and (b) above.
- ii) forming a culture comprising the cell identified in (i) above with an embryonic stem cell;
- and
- iii) growing said culture under conditions suitable for the maintenance and/or differentiation of said embryonic stem cell.
A method for modulating the differentiation of embryonic stem cells comprising:
- i) providing a cell transfected with a nucleic acid molecule selected from the group consisting of:
- a) a nucleic acid molecule as represented by the sequence in FIG. 22;
- b) a nucleic acid molecule which hybridises to the nucleic acid in (a) and which encodes a ligand capable of binding a Wnt receptor;
- and
- c) nucleic acid molecules which are degenerate as a result of the genetic code to the sequences defined in (a) and (b) above.
- ii) forming a culture comprising a cell identified in (i) above with an embryonic stem cell;
- and
- iii) growing said culture under conditions suitable for the maintenance and/or differentiation of embryonic stem cells.
A method for inhibiting the differentiation of embryonic stem cells comprising the steps of:
- i) providing at least one polypeptide, or active fragment thereof, wherein said polypeptide is an inhibitor of the Wnt signalling pathway.
- iii) forming a culture comprising the polypeptide identified in (i) above with an embryonic stem cell;
- and
- iii) growing said culture under conditions suitable for the maintenance of embryonic stem cells in an undifferentiated state.
A method for inhibiting the differentiation of embryonic stem cells comprising the steps of:
- i) providing a cell transfected with a nucleic acid molecule selected from the group consisting of:
- a) a nucleic acid molecule encoding a Wnt inhibitory polypeptide;
- b) a nucleic acid molecule which hybridises to the nucleic acid in (a) and which encodes a polypeptide capable of inhibiting Wnt signalling;
- and
- c) nucleic acid molecules which are degenerate as a result of the genetic code to the sequences defined in (a) and (b) above.
- ii) forming a culture of the cell identified in (i) above with an embryonic stem cell;
- and
- iii) growing said culture under conditions suitable for the maintenance of embryonic stem cells in an undifferentiated state.
Dependencies are inferred from the claim text.