Method for producing recombinant of methanol-assimilating bacterium
OwnerTakayuki Asahara, Hisashi Yasuedainventors
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Abstract
A recombinant of a methanol-assimilating bacterium in which an exogenous linear DNA fragment is introduced into its chromosomal DNA, and is prepared by the following steps: (a) preparing an exogenous linear DNA fragment comprising a nucleotide sequence identical to a nucleotide sequence of an arbitrary region of said chromosomal DNA, (b) introducing the linear DNA fragment into the methanol-assimilating bacterium to obtain recombinants, and (c) selecting a recombinant in which said region on the chromosome is replaced with said linear DNA fragment.
Claims5
A method for producing a recombinant of a methanol-assimilating bacterium in which an exogenous linear DNA fragment is introduced into the chromosomal DNA of the methanol-assimilating bacterium comprising:
- (a) preparing an exogenous linear DNA fragment comprising a nucleotide sequence identical to a nucleotide sequence of an arbitrary region of said chromosomal DNA,
- (b) introducing said linear DNA fragment into the methanol-assimilating bacterium to obtain recombinants, and
- (c) selecting a recombinant in which said region on the chromosome is replaced with said linear DNA fragment.
Dependencies are inferred from the claim text.