Novel glucose-6-phosphate dehydrogenase
AssigneeKyowa Hakko Kogyo Co., Ltd.
InventorsHaruhiko Yokoi, Seiko Ando, Keiko Ochiai, Yoshiyuki Yonetani, Shin-ichi Hashimoto
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Abstract
The present invention relates to a novel glucose-6-phosphate dehydrogenase (hereinafter referred to as “G6PD”) derived from a bacterium belonging to the genus Corynebacterium, a DNA encoding the enzyme, a recombinant DNA comprising the DNA, a transformant comprising the recombinant DNA, a transformant comprising the DNA on its chromosome, and a process for producing L-amino acid or G6PD which comprises culturing the transformant. According to the present invention, a modified G6PD and a DNA encoding the G6PD are obtained, and the productivity of L-amino acid by a microorganism can be improved by using the modified G6PD.
Claims23
A polypeptide which comprises the amino acid sequence represented by SEQ ID NO:2.
A polypeptide which comprises an amino acid sequence in which Ala at position 213 in the amino acid sequence represented by SEQ ID NO:2 is replaced with an other amino acid, and has glucose-6-phosphate dehyrdogenase activity.
A polypeptide which comprises the amino acid sequence represented by SEQ ID NO:12.
A polypeptide which comprises an amino acid sequence in which one or several amino acids other than the amino acid residue at position 213 in the amino acid sequence represented by SEQ ID NO:12 are deleted, substituted or added, and has glucose-6-phosphate dehyrdogenase activity.
A DNA which comprises the nucleotide sequence represented by SEQ ID NO:1.
A DNA which comprises a nucleotide sequence in which a nucleotide sequence of positions 637 to 639 encoding Ala in the nucleotide sequence represented by SEQ ID NO:1 is replaced with a codon encoding an amino acid other than Ala.
A DNA which comprises the nucleotide sequence represented by SEQ ID NO:11.
A DNA which hybridizes with a DNA comprising the nucleotide sequence represented by SEQ ID NO:1 under stringent conditions, and encodes a polypeptide having glucose-6-phosphate dehydrogenase activity, wherein a nucleotide sequence corresponding to the nucleotide sequence of positions 637 to 639 encoding Ala in the nucleotide sequence represented by SEQ ID NO:1 is replaced with a codon encoding an amino acid other than Ala.
A DNA which hybridizes with a DNA comprising the nucleotide sequence represented by SEQ ID NO:1 under stringent conditions, and encodes a polypeptide having glucose-6-phosphate dehyrdogenase activity, wherein a nucleotide sequence corresponding to the nucleotide of position 637 in the nucleotide sequence represented by SEQ ID NO:1 is replaced with adenine.
A plasmid pCRBzwfM comprised in Escherichia coli TOP10 (FERM BP-7135).
Dependencies are inferred from the claim text.