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US 2004/0171126 A1Published application
Process for the production of phospholipids
AssigneeEnzymotec Ltd.
InventorsSobhi Basheer, Rassan Zuabi, Avidor Shulman, Neta Scheinman
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AssigneeEnzymotec Ltd.
InventorsSobhi Basheer, Rassan Zuabi, Avidor Shulman, Neta Scheinman
Estimating the term…
A new enzymatic process for preparing 1,2-diacylated phospholipids using an enzyme preparation possessing phospholipase activity towards acylation at the sn-1 and sn-2 sites in a microaqueous reaction system. More particularly, the 1,2-diacyl-phospholipids produced according to the esterification/transesterification process are obtainable in high yield and purity and carry identical desired carboxylic acid, preferably fatty acid, acyl groups at the sn-1 and sn-2 positions. The process involves esterification/transesterification (acylation) of a glycerophospholipid, preferably glycerophosphoryl choline (GPC) with a desired carboxylic acid, preferably fatty acid, or their derivatives in the presence of the above mentioned appropriate enzyme preparation. The process of the invention further relates to a process for the production of 1-acyl-2-lyso-glycerophospholipid, preferably 2-lyso-PC by reacting glycerophospholipid, preferably glycerophosphoryl choline (GPC) with a desired carboxylic acid, preferably fatty acid, or their derivatives in the presence of a sn-1 specific phospholipase (PLA1 or PLA1,2) and a solvent, in a microaqueous medium.
A process for the production of 1,2-diacylated-glyceropospholipids and their synthetic or natural analogues, wherein the 1-acyl and 2-acyl groups are predetermined and identical, comprising contacting in a microaqueous environment a glycerophospholipid with a carboxylic acid acyl donor in the presence of a phospholipase enzyme capable of catalyzing an esterification/transesterificiation/acylation at both the sn-1 and sn-2 positions of said gylcerophospholipid.
A process for the production of 1,2-diacylated-glycerophospholipids, and their synthetic or natural analogues, in which the 1-and 2-acyl groups are identical and are predetermined, comprising contacting in a microaqueous environment a glycerophospholipid with a carboxylic acid acyl donor, preferably a fatty acid derivative capable of providing the same desired acyl group in the presence of a phospholipase enzyme capable of catalyzing an acylation at both the sn-1 and sn-2 positions of said glycerophospholipid.
A process for the production of 1-acylated-2-lyso-glycerophospholipids and their synthetic or natural analogues, wherein the 1-acyl group is predetermined, comprising contacting in a microaqueous environment a glycerophospholipid with a carboxylic acid acyl donor in the presence of a phospholipase capable of catalyzing an acylation at the sn-1 position (site) of said glycerophospholipid, in the presence of an organic solvent.
Dependencies are inferred from the claim text.