US 2004/0171119 A1Published application

Reductase gene and use of the same

AssigneeSumitomo Chemical Company, Limited

InventorNobuya Itoh

Life of this patent

Estimating the term…

Abstract

A gene containing a DNA having the nucleotide sequence encoding any one of the amino acid sequences of the following (a) to (e): (a) an amino acid sequence set out in SEQ ID NO: 1; (b) an amino acid sequence having the sequence homology of 80% or more with (a); (c) an amino acid sequence having the sequence homology of 90% or more with (a); (d) an amino acid sequence encoded by a DNA having the nucleotide sequence set out in SEQ ID NO: 2; (e) an amino acid sequence encoded by a DNA having the nucleotide sequence having the sequence homology of 80% or more with (d).

Claims22

Dependent claims

A gene comprising a DNA having the nucleotide sequence encoding any one of the amino acid sequences of the following

(a) to (h):
(a) an amino acid sequence set out in SEQ ID NO:
1;
(b) an amino acid sequence having the sequence homology of 80% or more with the amino acid sequence set out in SEQ ID NO:
1, and the amino acid sequence is an amino acid sequence of a protein having at least an ability to reduce 2,2,2-trifluoroacetophenone to 2,2,2-trifluoro-1-phenylethanol;
(c) an amino acid sequence having the sequence homology of 90% or more with the amino acid sequence set out in SEQ ID NO:
1, and the amino acid sequence is an amino acid sequence of a protein having at least an ability to reduce 2,2,2-trifluoroacetophenone to 2,2,2-trifluoro-1-phenylethanol;
(d) an amino acid sequence encoded by a DNA having the nucleotide sequence set out in SEQ ID NO:
2;
(e) an amino acid sequence encoded by a DNA having the nucleotide sequence having the sequence homology of 80% or more with a DNA having the nucleotide sequence set out in SEQ ID NO:
2, and the amino acid sequence is an amino acid sequence of a protein having at least an ability to reduce 2,2,2-trifluoroacetophenone to 2,2,2-trifluoro-1-phenylethanol;
(f) an amino acid sequence encoded by a DNA that hybridizes under the stringent condition with a DNA having a nucleotide sequence which is complementary to the nucleotide sequence set out in SEQ ID NO:
2, and the amino acid sequence is an amino acid sequence of a protein having at least an ability to reduce 2,2,2-trifluoroacetophenone to 2,2,2-trifluoro-1-phenylethanol;
(g) an amino acid sequence of a protein obtained from a microorganism belonging to genus Leifsonia, and the amino acid sequence is an amino acid sequence of a protein having at least an ability to reduce 2,2,2-trifluoroacetophenone to 2,2,2-trifluoro-1-phenylethanol;
and
(h) an amino acid sequence of a protein obtained from Leifsonia sp. S-749, and the amino acid sequence is an amino acid sequence of a protein having at least an ability to reduce 2,2,2-trifluoroacetophenone to 2,2,2-trifluoro-1-phenylethanol.

13 more elements

Dependencies are inferred from the claim text.

patentgrep

Loading patent…

Abstract

A gene comprising a DNA having the nucleotide sequence encoding any one of the amino acid sequences of the following

(a) to (h):
(a) an amino acid sequence set out in SEQ ID NO:
1;
(b) an amino acid sequence having the sequence homology of 80% or more with the amino acid sequence set out in SEQ ID NO:
1, and the amino acid sequence is an amino acid sequence of a protein having at least an ability to reduce 2,2,2-trifluoroacetophenone to 2,2,2-trifluoro-1-phenylethanol;
(c) an amino acid sequence having the sequence homology of 90% or more with the amino acid sequence set out in SEQ ID NO:
1, and the amino acid sequence is an amino acid sequence of a protein having at least an ability to reduce 2,2,2-trifluoroacetophenone to 2,2,2-trifluoro-1-phenylethanol;
(d) an amino acid sequence encoded by a DNA having the nucleotide sequence set out in SEQ ID NO:
2;
(e) an amino acid sequence encoded by a DNA having the nucleotide sequence having the sequence homology of 80% or more with a DNA having the nucleotide sequence set out in SEQ ID NO:
2, and the amino acid sequence is an amino acid sequence of a protein having at least an ability to reduce 2,2,2-trifluoroacetophenone to 2,2,2-trifluoro-1-phenylethanol;
(f) an amino acid sequence encoded by a DNA that hybridizes under the stringent condition with a DNA having a nucleotide sequence which is complementary to the nucleotide sequence set out in SEQ ID NO:
2, and the amino acid sequence is an amino acid sequence of a protein having at least an ability to reduce 2,2,2-trifluoroacetophenone to 2,2,2-trifluoro-1-phenylethanol;
(g) an amino acid sequence of a protein obtained from a microorganism belonging to genus Leifsonia, and the amino acid sequence is an amino acid sequence of a protein having at least an ability to reduce 2,2,2-trifluoroacetophenone to 2,2,2-trifluoro-1-phenylethanol;
and
(h) an amino acid sequence of a protein obtained from Leifsonia sp. S-749, and the amino acid sequence is an amino acid sequence of a protein having at least an ability to reduce 2,2,2-trifluoroacetophenone to 2,2,2-trifluoro-1-phenylethanol.

13 more elements