Process for producing dipeptides
AssigneeKyowa Hakko Kogyo Co., Ltd.
InventorsShin-ichi Hashimoto, Kazuhiko Tabata, Aya Kubota, Hajime Ikeda
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Abstract
The present invention provides a protein which catalyzes the synthesis of a dipeptide different from L-Ala-L-Ala, a process for producing the protein which catalyzes the synthesis of a dipeptide, a process for producing a dipeptide using the protein which catalyzes the synthesis of a dipeptide, and a process for producing the dipeptide using a culture of a microorganism producing the protein which catalyzes the synthesis of a dipeptide or the like as an enzyme source.
Claims20
A protein selected from the group consisting of [1] to [4] below, provided that a protein consisting of the amino acid sequence shown in SEQ ID NO:
- 1 is excluded:
- [1] a protein comprising the amino acid sequence shown in any of SEQ ID NOS:
- 2 to 8;
- [2] a protein comprising an amino acid sequence wherein one or more amino acid residues are deleted, substituted or added in the amino acid sequence shown in any of SEQ ID NOS:
- 1 to 8 and catalyzing the synthesis of a dipeptide represented by formula (I):
- R1—R2 (I)
- (wherein R1 and R2, which may be the same or different, each represent an amino acid, provided that both R1 and R2 cannot represent L-alanine at the same time);
- [3] a protein comprising an amino acid sequence which shows 65% or more similarity to the amino acid sequence shown in any of SEQ ID NOS:
- 1 to 8 and catalyzing the synthesis of dipeptide represented by formula (I);
- and
- [4] a protein comprising an amino acid sequence which shows 80% or more similarity to the amino acid sequence shown in SEQ ID NO:
- 17 and catalyzing the synthesis of a dipeptide represented by formula (I).
A nucleic acid sequence selected from the group consisting of [1] to [5] below, provided that the nucleotide sequence shown in SEQ ID NO:
- 9 is excluded, and the RNA equivalent thereof, are:
- [1] a nucleic acid sequence encoding the protein according to the above (1);
- [2] a nucleic acid sequence comprising the nucleotide sequence shown in any of SEQ ID NOS:
- 10 to 16 and 36;
- [3] a nucleic acid sequence which hybridizes with a nucleic acid sequence comprising the complement of a nucleotide sequence shown in any of SEQ ID NOS:
- 9 to 16 and 36 under stringent conditions and which nucleic acid sequence encodes a protein which catalyzes the synthesis of a dipeptide represented by formula (I):
- R1—R2 (I)
- (wherein R1 and R2, which may be the same or different, each represent an amino acid, provided that both R1 and R2 cannot represent L-alanine at the same time);
- [4] a nucleic acid sequence comprising a nucleotide sequence which shows 80% or more similarity to the nucleotide sequence shown in SEQ ID NO:
- 18 and encoding a protein which catalyzes the synthesis of a dipeptide represented by formula (I);
- and
- [5] a nucleic acid sequence which hybridizes with a nucleic acid sequence of any one of SEQ ID NOs:
- 9 to 16 and 36 under stringent conditions and is useful as a probe or primer for identifying, detecting, amplifying, etc. a nucleic acid sequence which catalyzes the synthesis of a dipeptide represented by formula (I).
Dependencies are inferred from the claim text.