Process for the production of a reversibly inactive acidified plasmin composition

A method for purifying plasmin comprising:

• cleaving a plasminogen using a catalytic concentration of a plasminogen activator to yield an active plasmin;
• binding the active plasmin to an active plasmin-specific absorbent material to form a bound plasmin;
• and
• eluting the bound plasmin with a substantially neutral pH excipient solution to form a final plasmin solution which is substantially free of degraded plasmin.

A process for the purification of plasminogen from a plasma source comprising:

• extracting plasminogen from a plasma paste fraction with a buffer solution at a pH in a range from about 3.5 to 10.5 and collecting the plasminogen-containing solution;
• adding polyethylene glycol, metal oxide, ammonium sulfate, or a combination thereof to the plasminogen-containing buffer solution to precipitate impurities;
• separating the precipitated impurities from the effluent containing plasminogen;
• and
• adding the effluent containing plasminogen to a plasminogen-specific absorbent material.