Apparatus and method for immunological labeling for thin tissue sections
AssigneeLeica Mikrosysteme GmbH
InventorHeinz Plank
Life of this patent
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Abstract
An apparatus and a method for immunological labeling of thin tissue sections are disclosed. The treatment fluid is applied in the form of liquid droplets onto at least one slide, the arrangement of liquid droplets corresponding to the arrangement of the thin tissue sections on the carrier plate. Each slide is retained in a transport container, the transport container being embodied in the form of a trough which comprises a peripheral delimiting wall that is closed off by a base. The base of one transport containers constitutes the cover of a transport container arranged beneath it. The transport containers are stacked in a first and a second station.
Claims26
An apparatus for immunological labeling of thin tissue sections, comprising:
- a carrier plate on which the thin tissue sections are affixed in a defined pattern;
- at least one slide on which a treatment liquid is applied in the form of droplets, wherein the arrangement of the droplets on the slide corresponds to the arrangement of the thin tissue sections on the carrier plate;
- a transport container for holding one slide;
- wherein the transport container has a peripheral delimiting wall attached to a base;
- and,
- a first and a second station, in which the transport containers are stacked, wherein the base of one transport container constitutes the cover of a transport container arranged beneath it.
A method for immunological labeling for thin tissue sections, comprising the steps of:
- a) creating an individual treatment process for the thin tissue sections, which are arranged on a carrier plate that is inserted in a treatment section;
- b) applying droplets onto a plurality of slides, each of which has multiple depressions for reception of the liquid droplets;
- c) placing the slides, together with a transport container provided for the slide, in a first station;
- d) transferring the transport container, together with the slide, from the first station to a treatment section by means of a transport mechanism;
- e) lifting the transport container, together with the slide, in such a way that the liquid droplets on the slide wet the thin tissue sections on the carrier plate;
- f) transferring the transport container together with the slide, at a time predetermined by the individual treatment process, to a second station that holds used slides;
- and
- g) performing steps d) through f) until all the slides provided in the first station for an individual treatment process have been removed.
Dependencies are inferred from the claim text.