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AssigneeThe Regents of the University of California
InventorsDennis J. Slamon, Marlene Schoenberg Fejzo
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The invention disclosed herein improves upon existing tissue microarray technology by using frozen tissues embedded in tissue embedding compound as donor samples and arraying the specimens into a recipient block comprising tissue embedding compound. Tissue is not fixed prior to embedding, and sections from the array are evaluated without fixation or post-fixed according to the appropriate methodology used to analyze a specific gene at the DNA, RNA, and/or protein levels. Unlike paraffin tissue arrays which can be problematic for immunohistochemistry and for RNA in situ hybridization analyses, the disclosed methods allow optimal evaluation by each technique and uniform fixation across the array panel. The disclosed arrays work well for DNA, RNA, and protein analyses, and have significant qualitative and quantitative advantages over existing methods.
A method of preparing a tissue microarray comprising the steps of:
A method of preparing a biological sample for microarray analysis comprising the steps of:
A method of preparing a tissue microarray comprising the steps of:
A process for preparing a biological sample for microarray analysis comprising embedding a non-fixed biological sample into an array within a block comprising frozen tissue embedding compound.
A composition comprising an array of biological samples comprising at least one non-fixed biological sample embedded in a tissue microarray block, wherein the tissue microarray block comprises frozen tissue embedding compound.
Dependencies are inferred from the claim text.