Method of detecting inorganic phosphoric acid, pyrophosphate and nucleic acid, and method of typing SNP sequence of DNA

A method of detecting a pyrophosphate which comprises:

• converting the pyrophosphate in a sample into an inorganic phosphoric acid;
• subjecting said sample to a measurement system including glyceraldehyde-3-phosphate, oxidized nicotinamide adenine dinucleotide or oxidized nicotinamide adenine dinucleotide phosphate, glyceraldehyde phosphate dehydrogenase, and an electron mediator;
• and
• measuring a current value in said measurement system, wherein said current value indicates the concentration of the pyrophosphate in said sample.

A method of detecting a nucleic acid which comprises:

• subjecting a sample to a reaction system including a DNA probe having a complementary sequence to the sequence of said nucleic acid, DNA polymerase and a deoxynucleotide, and allowing extension of said DNA probe whereby a pyrophosphate produced concurrent with the extension reaction of said DNA probe;
• converting the pyrophosphate produced in the sample into an inorganic phosphoric acid;
• subjecting said sample to a measurement system including glyceraldehyde-3-phosphate, oxidized nicotinamide adenine dinucleotide or oxidized nicotinamide adenine dinucleotide phosphate, glyceraldehyde phosphate dehydrogenase, and an electron mediator;
• and
• measuring a current value in said measurement system, wherein said current value indicates the concentration of the nucleic acid having a specified sequence in said sample.

A method of typing a SNP sequence of a DNA which comprises:

• subjecting a sample to a reaction system including a DNA probe having a complementary sequence to the sequence of said DNA and having a SNP site, DNA polymerase and a deoxynucleotide, and allowing extension of said DNA probe whereby a pyrophosphate produced concurrent with the extension reaction of said DNA probe;
• converting the pyrophosphate produced in the sample into an inorganic phosphoric acid;
• subjecting said sample to a measurement system including glyceraldehyde-3-phosphate, oxidized nicotinamide adenine dinucleotide or oxidized nicotinamide adenine dinucleotide phosphate, glyceraldehyde phosphate dehydrogenase, and an electron mediator;
• and
• measuring a current value in said measurement system, wherein said current value indicates the presence of the DNA having a specified sequence in said sample.