Oxygen sensors disposed on a microtiter plate
OwnerIngo Klimant, Christian Krauseinventors
Life of this patent
Estimating the term…
Abstract
The invention relates to a novel sensor wherein the micro titer plates or supports are fitted with wells receiving the specimens to ascertain oxygen content. The wells contain luminescent or fluorescent dyes (for instance platinum, palladium or ruthenium complexes with phenathroline, porphyrin or pyridine ligands) which are imbedded in the particles of a gas-permeable but water-impermeable matrix. The matrix is a polystyrene derivative or a polystyrene copolymer. The particles in turn are dispersed in a second, water-permeable matrix consisting of a hydrophilic polymer such as polyhydroxy methacrylate, polyvinyl alcohol or polyvinyl pyrrolidone.
Claims15
Oxygen-detecting system comprising:
- a support fitted with several wells receiving specimens and oxygen sensors, said sensors comprising
- (a) particles which contain
- (i) a first luminescent indicator dye quenchable by oxygen, and
- (ii) a gas-permeable, substantially water-impermeable first matrix, and
- (b) a substantially water-permeable second matrix, the particles (a) being dispersed
- in the second matrix (b), and
- (c) a reference dye which is spectrally different from the first dye and substantially oxygen-insensitive, the indicator dye's sensitivity being higher by a factor≧10 than the sensitivity of the reference dye.
Dependencies are inferred from the claim text.