Method for counting leukocytes and leukocyte counter
AssigneeARKRAY, Inc.
InventorsAkio Okubo, Shigeki Yamada
Life of this patent
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Abstract
A method for counting leukocytes which comprises liberating elastase from granulocytes contained in a specimen, adding an anti-granulocyte elastase antibody to the thus liberated elastase, measuring the antibody bonded to the elastase to thereby determine the concentration of the elastase, and then calculating therefrom the number of leukocytes contained in the specimen with the use of the ratio of the leukocyte count to the elastase concentration. This method makes it possible to conveniently and less expensively count leukocytes at a high accuracy comparable to the one established by using a conventional automatic blood cell counter, as the figure shows.
Claims22
A method for counting leukocytes comprising:
- liberating elastase from neutrophils, eosionphils and basophils (hereafter, these three blood cells are referred to as “granulocytes”) in leukocytes contained in a specimen;
- adding an antigranulocyte elastase antibody to the thus liberated elastase;
- measuring the antigranulocyte elastase antibody bonded to the elastase to thereby determine the concentration of the elastase;
- and calculating the number of leukocytes contained in the specimen from the thus determined concentration of elastase by using a known ratio of the leukocyte count, the granulocyte count or the neutrophil count to the concentration of elastase.
Dependencies are inferred from the claim text.