Screening compound libraries using an optical fiber array device capable of simultaneously performing multiple functional assays
OwnerKimon Angelidesinventor
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Abstract
Disclosed is a method of screening Compounds wherein target cells are coated onto a population of microbeads, and wherein each microbead is coated with several cells of the same cellular type and has an assay and an assay reporter associated with it. Each of the cell-coated microbeads are positioned in a well formed in one end of a fiber which is part of an array of optical fibers, and the microbeads are contacted with the compounds. The results of the assay associated with a microbead are reported to the distal end of the fiber, and each fiber in the array so reports.
Claims25
A method of screening Compounds comprising:
- coating target cells onto a population of microbeads, wherein each microbead is coated with several cells of the same cellular type and has an assay and an assay reporter associated with it;
- positioning each of the cell-coated microbeads in a well formed in one end of a fiber which is part of an array of optical fibers;
- contacting the microbeads with Compounds;
- and
- reporting the results of the assay associated with a microbead to the distal end of the fiber.
A method of screening Compounds comprising:
- coating target cells onto a population of microbeads, wherein each microbead is coated with several cells of the same cellular type and has an assay and an assay reporter associated with it;
- positioning each of the cell-coated microbeads in a well formed in one end of a fiber in an array of optical fibers;
- placing a series of Compounds into a series of microtiter plate wells;
- positioning the end of the array with the microbeads into one of the microtiter plate wells;
- and
- reporting the results of the assays associated with microbeads to the distal end of the array of fibers.
A method of screening Compounds comprising:
- coating two or more different types of target cells onto a population of microbeads, wherein each microbead is coated with several cells of the same cellular type, is encoded to identify the cellular type it is coated with, and has an assay and an assay reporter associated with it;
- positioning each of the cell-coated microbeads in a particular well formed in one end of a particular fiber in an array of optical fibers;
- placing a series of Compounds into a series of wells;
- positioning the end of the array with the microbeads sequentially into the wells;
- and
- reporting the results of the assays associated with microbeads to the distal end of the array of fibers.
A method of screening Compounds comprising:
- coating target cells onto a population of microbeads, wherein each microbead is coated with several cells of two or more different cellular types, wherein each cellular type has an assay and an assay reporter associated with it, and each cellular type is encoded to indicate which cellular type it is;
- positioning each of the cell-coated microbeads into a well formed in one end of a fiber in an array of optical fibers;
- placing a series of Compounds into a series of assay plate wells;
- positioning the end of the array with the microbeads sequentially into the assay plate wells;
- and
- reporting the results of the assays associated with microbeads to the distal end of the array of fibers.
Dependencies are inferred from the claim text.