Antigens
OwnerAbraham Mittelman, Darja Kanducinventors
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Abstract
The invention provides a method for identifying amino acid sequences in antigens of interest that are useful for evoking immune responses. The amino acid sequences have low sequence similarity to the host proteome and are predicted to bind to MHC. Also disclosed are HPV epitopes that evoke Class I or Class II mediated immune responses.
Claims19
A method of identifying an immunodominant epitope of an antigen comprising:
- examining amino acid sequences within the antigen for binding affinity to an MHC molecule;
- examining amino acid sequences within the antigen to determine sequence similarity to the host proteome;
- and
- selecting an amino acid sequence within the antigen having high MHC binding affinity and low sequence similarity to the host proteome.
A method of producing a polypeptide useful for eliciting an immune response against an antigen in a host comprising;
- (a) analyzing amino acid sequences within the antigen for binding affinity to an MHC molecule;
- (b) examining amino acid sequences within the antigen to determine sequence similarity to the host proteome;
- (c) selecting an amino acid sequence having high MHC binding affinity and low sequence similarity;
- and
- (d) producing a polypeptide comprising the selected amino acid sequence.
A method of eliciting a therapeutic immune response to an antigen comprising administering to a subject an effective amount a polypeptide comprising an amino acid selected by:
- (a) analyzing amino acid sequences within the antigen for binding affinity to an MHC molecule;
- (b) examining amino acid sequences within the antigen to determine sequence similarity to the host proteome;
- and
- (c) selecting an amino acid sequence having high MHC binding affinity and low sequence similarity.
Dependencies are inferred from the claim text.