Screening method

A kit for screening a compound or salts thereof that alter the binding property between a G protein-coupled receptor protein or salts thereof, and a substance relating to cholesterol metabolism, which comprises using (1) the receptor protein containing the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO:

• 1, a partial peptide of the receptor protein or salts thereof, and (2) the substance relating to cholesterol metabolism.

A prophylactic and/or therapeutic agent for inflammatory diseases or hyper immunoreaction after implantation comprising a compound or salts thereof that increase an expression level of the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1.

A prophylactic and/or therapeutic agent for immunodeficiency diseases or infectious diseases comprising a compound or salts thereof that reduce an expression level of the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1.

A method of prophylaxis and/or therapy for inflammatory diseases or hyper immunoreaction after implantation, which comprises administering an effective dose of the compound or salts thereof that increase an expression level of the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1, to mammals.

A method of prophylaxis and/or therapy for immunodeficiency diseases or infectious diseases, which comprises administering an effective doses of the compound or salts thereof that reduce an expression level of the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1, to mammals.

Use of the compound or salts thereof that increase an expression level of the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1, for manufacturing a prophylactic and/or therapeutic agent for inflammatory diseases or hyper immunoreaction after implantation.

Use of the compound or salts thereof that reduce an expression level of the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1, for manufacturing a prophylactic and/or therapeutic agent for immunodeficiency diseases or infectious diseases.

A prophylactic and/or therapeutic agent for inflammatory diseases or hyper immunoreaction after implantation comprising the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1, its partial peptide, or salts thereof

A prophylactic and/or therapeutic agent for inflammatory diseases or hyper immunoreaction after implantation comprising a polynucleotide containing the polynucleotide encoding the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1 or its partial peptide.

A diagnostic for central diseases, inflammatory diseases, circuratory diseases, canser, respiratory diseases, diabetes, immune system disorders, diseases of liver and gallbladder, alimentary diseases, infectious diseases, adiposis or hyper immunoreaction after implantation comprising a polynucleotide containing the polynucleotide encoding the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1 or its partial peptide.

A prophylactic and/or therapeutic agent for immunodeficiency diseases or infectious diseases comprising an antibody against the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1, its partial peptide, or salts thereof.

A diagnostic for central diseases, inflammatory diseases, circuratory diseases, canser, respiratory diseases, diabetes, immune system disorders, diseases of liver and gallbladder, alimentary diseases, infectious diseases, adiposis or hyper immunoreaction after implantation comprising an antibody against the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1, its partial peptide, or salts thereof.

A prophylactic and/or therapeutic agent for immunodeficiency diseases or infectious diseases comprising an antisense polynucleotide containing a base sequence complementary to a polynucleotide containing the polynucleotide encoding the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1 or its partial peptide, or a portion thereof.

A diagnostic for central diseases, inflammatory diseases, circuratory diseases, canser, respiratory diseases, diabetes, immune system disorders, diseases of liver and gallbladder, alimentary diseases, infectious diseases, adiposis or hyper immunoreaction after implantation comprising an antisense polynucleotide containing a base sequence complementary to a polynucleotide containing the polynucleotide encoding the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1 or its partial peptide, or a portion thereof.

A method of prophylaxis and/or therapy for inflammatory diseases or hyper immunoreaction after implantation, which comprises administering an effective dose of the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1, its partial peptide, or salts thereof, to mammals.

A method of prophylaxis and/or therapy for inflammatory diseases or hyper immunoreaction after implantation, which comprises administering an effective dose of the polynucleotide containing the polynucleotide encoding the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1 or its partial peptide, to mammals.

A method of prophylaxis and/or therapy for immunodeficiency diseases or infectious diseases, which comprises administering an effective dose of the antibody against the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1, its partial peptide, or salts thereof, to mammals.

A method of prophylaxis and/or therapy for immunodeficiency diseases or infectious diseases, which comprises administering an effective dose of the antisense polynucleotide containing a base sequence complementary to a polynucleotide containing the polynucleotide encoding the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1 or its partial peptide, or a portion thereof, to mammals.

Use of the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1, its partial peptide, or salts thereof, for manufacturing a prophylactic and/or therapeutic agent fot inflammatory diseases or hyper immunoreaction after implantation.

Use of the polynucleotide containing the polynucleotide encoding the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1 or its partial peptide, for manufacturing a prophylactic and/or therapeutic agent fot inflammatory diseases or hyper immunoreaction after implantation.

Use of the antibody against the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1, its partial peptide, or salts thereof, for manufacturing a prophylactic and/or therapeutic agent fot immunodeficiency diseases or infectious diseases.

Use of the antisense polynucleotide containing a base sequence complementary to a polynucleotide containing the polynucleotide encoding the G protein-coupled receptor protein containing the same or substantially the same amino acid sequence represented by SEQ ID NO:

• 1 or its partial peptide, or a portion thereof, for manufacturing a prophylactic and/or therapeutic agent fot immunodeficiency diseases or infectious diseases.

A method of determining a ligand to the receptor protein, which comprises adding a test compound to animal cells expressing a receptor protein, which the ligand is not determined, and containing a plasmid, which a DNA encoding reporter protein is ligated downstream cAMP response element/promoter, and measuring an activity of the reporter protein.

The method according to 68, wherein the similar characteristics is a basic expression level of the reporter protein and (or) an expression level of the reporter protein at the time of adding forskolin.