Method for expression of proteins on spore surface

A method for improving a protein of interest, which comprises the steps of:

• (i) constructing a gene library of the protein of interest;
• (ii) preparing a vector by linking the gene library to a gene encoding spore coat protein;
• (iii) transforming a spore-forming host cell with the vector;
• (iv) forming a spore in the transformed host cell and displaying the protein of interest on a surface of the spore;
• (v) recovering the spore displaying on its surface the protein of interest;
• and
• (vi) screening the spore displaying a variant of the protein of interest having a desired property.

A method for improving a protein of interest using a resistance property of spore, which comprises the steps of:

• (i) constructing a gene library of the protein of interest;
• (ii) preparing a vector by linking the gene library to a gene encoding spore coat protein;
• (iii) transforming a spore-forming host cell with the vector;
• (iv) forming a spore in the transformed host cell and displaying the protein of interest on a surface of the spore;
• (v) treating the spore displaying on its surface the protein of interest with one or more selected from the group consisting of organic solvent, heat, acid, base, oxidant, dryness, surfactant and protease;
• (vi) recovering the spore displaying on its surface the protein of interest;
• and
• (vii) screening the spore displaying a variant of the protein of interest having a resistance to the treatment.

A method for bioconversion, which comprises the steps of:

• (i) preparing a vector for spore surface display comprising a gene construct containing a gene encoding spore coat protein and a gene encoding a protein of interest conducting a bioconversion reaction, wherein, when expressed, the gene construct expresses a fusion protein between the spore coat protein and the protein of interest;
• (ii) transforming a host cell with the vector for spore surface display;
• (iii) displaying the protein of interest on a surface of a spore of the host cell;
• (iv) recovering the spore displaying on its surface the protein of interest;
• and
• (v) performing the bioconversion reaction using the spore displaying on its surface the protein of interest.

A method for preparing protein microarray, which comprises the steps of:

• (i) preparing a vector for spore surface display comprising a gene construct containing a gene encoding spore coat protein and a gene encoding antibody or antigen having binding affinity to a protein to be analyzed, wherein, when expressed, the gene construct expresses a fusion protein between the spore coat protein and the antibody or antigen;
• (ii) transforming a host cell with the vector for spore surface display;
• (iii) displaying the antibody or antigen on a surface of a spore of the host cell;
• (iv) recovering the spore displaying on its surface the antibody or antigen;
• and
• (v) immobilizing onto a solid surface the spore displaying on its surface the antibody or antigen.

A method producing an antibody to antigen in vertebrates, which comprises the steps of:

• (i) preparing a vector for spore surface display comprising a gene construct containing a gene encoding spore coat protein and a gene encoding the antigen, wherein, when expressed, the gene construct expresses a fusion protein between the spore coat protein and the antigen;
• (ii) transforming a host cell with the vector for spore surface display;
• (iii) displaying the antigen on a surface of a spore of the host cell;
• (iv) recovering the spore displaying on its surface the antigen;
• and
• (v) administering to vertebrates a composition containing an immunologically effective amount of the spore displaying on its surface the antigen.

A method for preparing a whole cell absorber, which comprises the steps of:

• (i) preparing a vector for spore surface display comprising a gene construct containing a gene encoding spore coat protein and a gene encoding a protein having a binding affinity to a certain substance, wherein, when expressed, the gene construct expresses a fusion protein between the spore coat protein and the protein;
• (ii) transforming a host cell with the vector for spore surface display;
• (iii) displaying the protein on a surface of a spore of the host cell;
• (iv) recovering the spore displaying on its surface the protein;
• and
• (v) immobilizing onto a carrier the spore displaying on its surface the protein.