Electro-optical devices and methods for hybridization and detection
AssigneeAgilent Technologies, Inc.
InventorsGanapati R. Mauze, Dan-Hui Yang
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Abstract
The invention provides an apparatus and method for detection of a target molecule. The apparatus includes a probe labeled with a transition metal-ligand complex that hybridizes with the target to form an initial complex, a metal ion for doping the initial complex and forming a final complex, and a potential means for providing a potential to the final complex to produce a detectable signal indicating the presence of the target after redox reaction. The method of the invention teaches the steps of hybridizing a probe with an attached label to the target to produce an initial complex, adding a metal ion to the initial complex to form a final complex and applying a potential to the final complex to produce a measurable signal.
Claims21
1 An apparatus for detecting the presence of a target, comprising:
- (a) a probe labeled with a transition metal-ligand complex, said probe for hybridizing with said target to form an initial complex;
- (b) a metal ion for doping the initial complex and forming a final complex;
- and
- (c) potential means for providing a potential to said final complex to produce a detectable signal indicating the presence of said target.
An apparatus as recited in claim 1, wherein said transition metal-ligand complex is selected from the group consisting of ruthenium, osmium and their derivatives.
An apparatus as recited in claim 1, wherein said metal ion for doping said initial complex is selected from the group consisting of zinc, nickel and cobalt.
An apparatus as recited in claim 1, wherein said final complex is conductive.
An apparatus as recited in claim 1, wherein said target and probe have become bound through hybridization.
An apparatus as recited in claim 1, wherein said signal is a chemiluminescent signal.
A method for detecting the presence of a target, comprising:
- (a) hybridizing a probe with an attached label to said target to produce an initial complex;
- (b) adding a metal ion to the initial complex to produce a final complex;
- and
- (c) applying a potential to the final complex to produce a measurable signal.
A method for detecting the presence of a target, comprising adding together a probe having an attached label, a target capable of hybridizing to the probe, and metal ions.
A method for detecting the presence of a target, comprising:
- (a) hybridizing a probe having an attached label with said target to produce an initial complex, wherein the label produces a signal in response to application of a potential;
- (b) adding a metal ion to the initial complex to produce a final electrically conductive complex;
- and
- (c) applying the potential through the final complex to the label, to cause the label to produce the signal.
A kit for detecting the presence of a target, comprising:
- (a) a probe having an attached label that hybridizes to said target to produce an initial complex, wherein the-label produces a signal in response to application of a potential;
- (b) a metal ion for adding to the initial complex to produce a final electrically conductive complex;
- and
- (c) potential means for applying a potential through said final complex to said label, to cause the label to produce a signal.
Dependencies are inferred from the claim text.