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AssigneeIsoTis N.V.
InventorsJoost Dick de Bruijn, Sandra Claudia da Silva Madureira Mendes, Clemens Antoni van Blitterswijk
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The invention relates to a method of determining in vitro the capacity of a cell population to induce bone formation in vivo comprising the steps of: a) providing a sample of a cell population; b) dividing said sample into a first and a second part containing an equal number of cells; c) culturing the first part in the presence of an osteogenic stimulation factor; d) culturing the second part in the absence of an osteogenic stimulation factor; e) determining degrees of expression of a bone-specific protein; and f) comparing the degrees of expression of the bone-specific protein of the first part and the second part thereby providing a measure for the capacity of the bone cell population to induce bone formation in vivo.
A method for determining in vitro the capacity of a cell population to induce bone formation in vivo comprising the steps of:
A method according to any of the preceding claims, wherein the osteogenic stimulation factor is dexamethasone or vitamin D3.
A method according to any of the preceding claims, wherein the cells are cultured for 2 to 15 doubling times.
A method according to any of the preceding claims, wherein the cells are cultured in a culture medium based on α-MEM.
A method according to any of the preceding claims, wherein the bone-specific protein is chosen from the group of alkaline phosphatase, osteocalcine, bone sialo protein, osteopontine and osteonectine.
Dependencies are inferred from the claim text.