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AssigneeSenomyx, Inc.
InventorsJon Elliot Adler, Sergey Zozulya, Xiadong Li, Shawn O'Connell, Lena Staszewski
Estimating the term…
Newly identified mammalian taste-cell-specific G protein-coupled receptors, and the genes and cDNA encoding said receptors are described. Specifically, T1R G protein-coupled receptors active in taste signaling, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for representing taste perception of a particular tastant in a mammal are also described, as are methods for generating novel molecules or combinations of molecules that elicit a predetermined taste perception in a mammal, and methods for simulating one or more tastes. Further, methods for stimulating or blocking taste perception in a mammal are also disclosed.
An isolated nucleic acid selected from the group consisting of:
An isolated genomic DNA molecule consisting essentially of a nucleic acid sequence coding for a mammalian G protein-coupled receptor polypeptide, wherein said nucleic acid sequence consists essential of SEQ ID NO 15 or 20.
An isolated genomic DNA molecule consisting essentially of a nucleic acid sequence coding for a mammalian G protein-coupled receptor having an amino acid sequence selected from the group consisting of SEQ ID NOs 4, 14, and 17.
An isolated genomic DNA molecule consisting essentially of a nucleic acid sequence having at least about 50% identity to a nucleic acid sequence coding for a mammalian G protein-coupled receptor polypeptide, wherein said nucleic acid sequence consists essentially of a nucleic acid sequence selected from the group consisting of SEQ ID NOs 15 and 20.
An isolated genomic DNA molecule consisting essentially of a nucleic acid sequence coding for a mammalian G protein-coupled receptor having an amino acid sequence that is at least about 40% identical to the amino acid sequence selected from the group consisting of SEQ ID NO:
An isolated cDNA molecule comprising a nucleic acid sequence having the same sequence as the mammalian G protein-coupled receptor polypeptide coding region contained in a genomic DNA sequence consisting essentially of a nucleic acid sequence selected from the group consisting of SEQ ID NOs 15 and 20.
An isolated cDNA molecule comprising a nucleic acid sequence coding for a G protein-coupled receptor polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs 4, 14, and 17.
An isolated cDNA molecule comprising a nucleic acid sequence having at least about 50% sequence identity to the G protein-coupled receptor polypeptide coding region in a genomic DNA sequence consisting essentially of a nucleic acid sequence selected from the group consisting of SEQ ID NOs 15 and 20.
An isolated cDNA molecule comprising a nucleic acid sequence having at least about 40% sequence identity to a sequence encoding a mammalian T1R G protein-coupled receptor polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs 4, 10, 12, 14, and 17.
An isolated variant DNA molecule comprising a nucleotide sequence consisting essentially of a nucleic acid sequence selected from the group consisting of SEQ ID NOs 15 and 20, containing at least one conservative substitution in a region coding for a G protein-coupled receptor active in taste signaling.
An isolated variant molecule comprising a nucleotide sequence encoding a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs 4, 14, and 17, containing at least one conservative substitution in a coding region.
An isolated nucleic acid molecule encoding a G protein-coupled receptor polypeptide active in taste signaling having the nucleotide sequence of SEQ ID NO:
An isolated nucleic acid molecule encoding a G protein-coupled receptor polypeptide active in taste signaling having the nucleotide sequence of SEQ ID NO:
An isolated nucleic acid molecule encoding a G protein-coupled receptor polypeptide active in taste signaling having the nucleotide sequence of SEQ ID NO:
An isolated nucleic acid molecule encoding a G protein-coupled receptor polypeptide active in taste signaling having the nucleotide sequence of SEQ ID NO:
An isolated nucleic acid molecule having the nucleotide sequence of SEQ ID NO:
An isolated nucleic acid molecule having the nucleotide sequence of SEQ ID NO:
An isolated nucleic acid molecule having the nucleotide sequence of SEQ ID NO:
An isolated nucleic acid molecule having the nucleotide sequence of SEQ ID NO:
An isolated nucleic acid molecule encoding the polypeptide having the amino acid sequence of SEQ ID NO:
An isolated nucleic acid molecule encoding the G protein-coupled receptor polypeptide active in taste signaling having the amino acid sequence of SEQ ID NO:
An isolated nucleic acid molecule encoding the G protein-coupled receptor polypeptide active in taste signaling having the amino acid sequence of SEQ ID NO:
An isolated nucleic acid molecule encoding the polypeptide having the amino acid sequence of SEQ ID NO:
An isolated nucleic acid molecule encoding the polypeptide having the amino acid sequence of SEQ ID NO:
An isolated nucleic acid molecule encoding a G protein-coupled receptor polypeptide active in taste signaling comprising the consensus sequence of SEQ ID NO:
An isolated nucleic acid encoding a G protein-coupled receptor polypeptide active in taste signaling comprising the consensus sequence of SEQ ID NO:
A genomic DNA amplified by a PCR reaction with at least one degenerate primer having a nucleic acid sequence of SEQ ID NOs 5 or 6, or consisting essentially of a nucleic acid sequence encoding a consensus sequence of SEQ ID NO 18 or 19, wherein said amplified DNA comprises a coding sequence for a G protein-coupled receptor polypeptide active in taste signaling.
A method for isolating a genomic sequence comprising a coding sequence for a G protein-coupled receptor polypeptide active in taste signaling, said method comprising contacting a mammalian genome with at least one degenerate primer having a nucleic acid sequence of SEQ ID NOs 5 or 6, or consisting essentially of a nucleic acid sequence encoding a consensus sequence of SEQ ID NO 18 or 19, and amplifying said genomic sequence comprising said primer sequence in the presence of polymerase, free nucleotides and cofactors.
A method for screening a mammalian genome for a coding sequence for a G protein-coupled receptor active in taste signaling, comprising:
Plasmid SAV115 comprising a mouse T1R3 gene.
Plasmid SAV118 comprising a rat T1R3 gene.
An isolated polypeptide selected from the group consisting of:
An isolated polypeptide having the amino acid sequence of SEQ ID NO:
An isolated polypeptide having the amino acid sequence of SEQ ID NO:
An isolated polypeptide having the amino acid sequence of SEQ ID NO:
An isolated polypeptide having the amino acid sequence of SEQ, ID NO:
An isolated polypeptide having the amino acid sequence of SEQ ID NO:
A method for representing the perception of one or more tastes in one or more mammals, comprising the steps of:
A method for predicting the taste perception in a mammal generated by one or more molecules or combinations of molecules comprising the steps of:
A genomic DNA molecule consisting essentially of a nucleic acid sequence coding for a mammalian G protein-coupled receptor polypeptide active in taste signaling comprising a consensus sequence selected from the group consisting of SEQ ID NOs 18 and 19, and sequences having at least about 75% identity to SEQ ID NOs 18 or 19.
A cDNA sequence coding for a mammalian G protein-coupled receptor polypeptide active in taste signaling comprising a consensus sequence selected from the group consisting of SEQ ID NOs 18 and 19, and sequences having at least about 75% identity to SEQ ID NOs 18 or 19.
A cDNA molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs 3, 13, and 16.
A cDNA molecule comprising a nucleic acid sequence having at least about 50% identity to a sequence selected from the group consisting of SEQ ID NOs 3, 13, and 16.
An isolated nucleic acid molecule having the nucleic acid sequence of SEQ ID NO 20.
Dependencies are inferred from the claim text.