Levels of Pin1 in normal and cancerous tissue
AssigneePintex Pharmaceuticals, Inc.
InventorDa Gong Wang
Life of this patent
Estimating the term…
Abstract
Methods for the use of Pin1 as a marker of abnormal cell growth are disclosed. In another embodiment, the method includes evaluating the efficacy of a treatment of an abnormal cell growth, such as cancer, by monitoring the levels of Pin1 . The levels of Pin1 can be protein levels or nucleic acid levels.
Claims41
A method of detecting abnormal cell growth in a mammal, comprising assessing the level of Pin1 in a test sample from the mammal, wherein a decrease in the levels of Pin1 is indicative of abnormal cell growth.
A method of determining the stage of cancer in a test sample from a mammal, comprising assessing a level of Pin1 in the test sample, wherein the level of Pin1 correlates with the stage of the cancer.
A method of evaluating the efficacy of a treatment of abnormal cell growth in a mammal, comprising comparing a level of Pin1 in at least two test samples, wherein the test samples comprise a first test sample obtained at a first time and a second test sample obtained at a later second time, and comparing the level of Pin1 in the test samples to a control sample, wherein a trend toward the level of Pin1 in the control sample indicates the efficacy of the treatment of the abnormal cell growth in the mammal.
A method of claim 31 wherein, the method of detecting the level of Pin1 protein in a test sample from a mammal, comprises the steps of:
- contacting the test sample with an antibody having specificity for Pin1 under conditions suitable for binding of the antibody to Pin1 thereby resulting in the formation of a complex between the antibody and Pin1;
- detecting the complex between the antibody and Pin1;
- and
- comparing the amount of the complex in the test sample with an amount of a complex in a control sample, wherein a decrease in the amount of the complex between the antibody and Pin1 in the test sample compared to the complex in the control sample is indicative of abnormal cell growth.
A kit for determining the level of Pin1 in a test sample from a mammal comprising one or more reagents for detecting Pin1.
The Kit of claim 41, wherein one of the reagents is an antibody.
The kit of claim 41, wherein one of the reagents is a DNA probe.
The kit of claim 41, wherein one of the reagents is a control.
A method of treating an individual suffering from cancer comprising, administering to said individaul a Pin1 activator such that treatment occurs.
Dependencies are inferred from the claim text.