Methods and compositions for modulating P53 transcription factor
AssigneesIrm LLC, The Scripps Research Institute
InventorsQihong Huang, Sumit Chanda, John B. Hogenesch, Jeremy S. Caldwell
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Abstract
This invention provides novel p53 modulatory polypeptides. The invention also provides methods for screening modulators of p53. The methods comprise first screening test agents for modulators of a p53-modulatory polypeptide and then further screening the identified modulating agents for modulators of p53. The invention further provides methods and pharmaceutical compositions for modulating p53 bioactivities in a cell and for treating diseases and conditions mediated by abnormal cellular proliferation.
Claims28
A method for identifying an agent that modulates a p53 bioactivity, the method comprising:
- (a) assaying a biological activity of a p53-modulatory polypeptide encoded by a polynucleotide selected from the members listed in Table 1 and Table 2, or a fragment of said polypeptide, in the presence of a test agent to identify one or more modulating agents that modulate the biological activity of the polypeptide;
- and
- (b) testing one or more of the modulating agents for ability to modulate a p53 bioactivity.
A method for identifying an agent that modulates cellular level of p53, the method comprising:
- (a) assaying a biological activity of a p53-modulatory polypeptide encoded by a polynucleotide selected from the members listed in Tables 1 and 2, or a fragment of said polypeptide, in the presence of a test agent to identify a modulating agent that modulates the biological activity of the polypeptide;
- and
- (b) testing the modulating agent for ability to modulate cellular level of p53.
Dependencies are inferred from the claim text.