Multidrug resistance proteins in Drosophila and Anopheles
AssigneeInstitut Pasteur
InventorsCharles W. Roth, Paul T. Brey, Inge Holm, Marine Grailles, Andrey Rzhetsky
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Abstract
The invention provides, inter alia, isolated nucleic acid molecules that comprise nucleic acids encoding multiple drug resistance proteins from Drosophila melanogaster or Anopheles gambiae, herein referred to as dMRP or gMRP, respectively, vectors encoding dMRP or gMRP, and host cells transformed with vectors containing these nucleic acids
Claims11
A purified polynucleotide comprising a purified nucleotide sequence encoding a sequence selected from the group consisting of the SEQ ID NOS.:
- 1, 2, 3, 4, or 5, or a fragment thereof.
An isolated nucleic acid molecule that comprises nucleic acids encoding multiple drug resistance proteins from Drosophila melanogaster or Anopheles gambiae.
A method for determining the insecticidal MRP inhibition activity of a compound, which comprises:
- a) placing a culture of insect cells, transformed with a vector capable of expressing dMRP or gMRP, in the presence of:
- (i) an insecticidal agent to which said insect cell is resistant, but to which said insect cell is sensitive in its untransformed state;
- (ii) a compound suspected of possessing insecticidal MRP inhibition activity;
- and
- b) determining the insecticidal MRP inhibition activity of said compound by measuring the ability of the insecticidal agent to inhibit the growth of said insect cell.
A strain of Drosophila melanogaster or Anopheles gambiae in which the dMRP or gMRP gene is disrupted or otherwise mutated such that the dMRP or gMRP protein is not produced in said strain.
Dependencies are inferred from the claim text.