Loading patent…
AssigneeArizona Board of Regents on Behalf of the University of Arizona
InventorsScot W. Ebbinghaus, Laurence H. Hurley, Adam Siddiqui-Jain, Regan Memmott
Estimating the term…
Among the different intrastrand quadruplex structures that can arise from duplex DNA, it has been discovered that the nucleotide sequences (GGA)4 and (GGA)3GG form biologically significant quadruplex structures. Thus, provided herein are methods for identifying molecules that modulate the biological activity of quadruplex DNA comprising the nucleotide sequence (GGA)4 or the nucleotide sequence (GGA)3GG, and specifically, methods for identifying molecules that bind such quadruplexes. Also provided herein are methods for modulating the biological activity of a biologically significant native quadruplex DNA with a molecule identified by the methods described herein.
A method for identifying a molecule that modulates the biological activity of a native quadruplex DNA, which comprises
A method for modulating the biological activity of a biologically significant native quadruplex DNA, which comprises contacting a system comprising the native quadruplex DNA with a molecule that interacts with quadruplex DNA comprising the nucleotide sequence (GGA)4 or the nucleotide sequence (GGA)3GG, wherein G is guanine and A is adenine;
A method for identifying a quadruplex structure in a nucleic acid of a sample, which comprises contacting the sample with a quadruplex-interacting agent and detecting the presence or absence of an interaction between the nucleic acid and the quadruplex-interacting agent, whereby the presence of an interaction is indicative of the quadruplex structure in the nucleic acid.
A method for identifying a nucleotide sequence capable of forming a quadruplex structure, which comprises identifying in a database a subset of nucleotide sequences comprising (GGA)4, (GGA)3GG or (GGA)3GGXn(GGA)3GG, wherein n is an integer between 0 and 3.
A method for identifying a nucleotide sequence capable of forming a quadruplex structure, which comprises contacting a cell with a quadruplex interacting agent, identifying a subset of RNA nucleotide sequences increased or decreased 2-fold of more in the cell as compared to a cell not contacted with the quadruplex interacting agent, and identifying a nucleotide sequence from the subset comprising (GGA)4, (GGA)3GG or (GGA)3GGXn(GGA)3GG as the nucleotide sequence capable of forming a quadruplex structure.
Dependencies are inferred from the claim text.