Method of efficiently detecting double-stranded nucleic acid

A method for reducing signals derived from an intercalator bound to a single-stranded nucleic acid, wherein a compound that is bound to a single-stranded nucleic acid more strongly than an intercalator and is bound to a double-stranded nucleic acid more weakly than an intercalator is added to a mixture comprising double-stranded and single-stranded nucleic acids.

A method for detecting a product of nucleic acid amplification comprising the following steps:

• (a) amplifying a nucleic acid through nucleic acid amplification in the presence of an intercalator and a compound that is bound to a single-stranded nucleic acid more strongly than an intercalator and is bound to a double-stranded nucleic acid more weakly than an intercalator;
• and
• (b) assaying the fluorescence intensity of a reaction solution.

A kit for detecting double-stranded nucleic acids comprising, as elements, an intercalator and a compound that reacts more preferentially with an intercalator bound to a single-stranded nucleic acid than with an intercalator bound to a double-stranded nucleic acid and/or a compound that is bound to a single-stranded nucleic acid more strongly than an intercalator and is bound to a double-stranded nucleic acid more weakly than an intercalator.